Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_23745 and PS417_23755 are separated by 1351 nucleotidesPS417_23755 and PS417_23760 are separated by 4 nucleotidesPS417_23760 and PS417_23765 overlap by 4 nucleotidesPS417_23765 and PS417_23770 are separated by 142 nucleotides PS417_23745: PS417_23745 - hypothetical protein, at 5,133,390 to 5,134,238 _23745 PS417_23755: PS417_23755 - membrane protein, at 5,135,590 to 5,136,081 _23755 PS417_23760: PS417_23760 - DeoR faimly transcriptional regulator, at 5,136,086 to 5,137,348 _23760 PS417_23765: PS417_23765 - hypothetical protein, at 5,137,345 to 5,137,920 _23765 PS417_23770: PS417_23770 - exodeoxyribonuclease V subunit alpha, at 5,138,063 to 5,140,132 _23770
Group Condition PS417_23745 PS417_23755 PS417_23760 PS417_23765 PS417_23770
ecofab LB in EcoFAB_3.5mL -0.1 -0.8 -0.9 -0.4 -3.1
stress methylglyoxal 0.032 vol% -0.1 -0.5 +0.3 -1.3 -3.7
stress Gentamicin 0.008 mg/ml -0.1 +0.4 -0.4 -1.3 -3.5
soil soil sample 3; outgrowth in LB -0.2 -0.6 -0.8 -0.4 -2.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 -0.5 +0.0 -1.5 -2.7
ecofab LB 0.5x in EcoFAB_3.5mL +0.0 -1.2 -0.1 -0.2 -3.0
soil soil sample 5; outgrowth in LB +0.1 -0.4 -0.4 -0.9 -2.9
stress Benzalkonium Chloride 0.012 mg/ml -0.1 -0.6 -0.2 +0.1 -3.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.2 -0.6 -0.2 -0.4 -3.2
soil soil sample 6; outgrowth in LB +0.1 -2.1 +0.1 +0.0 -2.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -1.9 +0.4 -0.0 -2.6
stress Spectinomycin 0.4 mg/ml -0.1 +0.5 -0.2 -0.0 -4.2
stress Nalidixic 0.025 mg/ml -0.0 +0.2 +0.3 +0.1 -4.6
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB +0.1 -0.5 +0.1 +0.2 -3.6
motility outer cut, LB soft agar motility assay -0.0 -0.9 +0.1 +0.2 -3.0
stress Nalidixic 0.015 mg/ml +0.1 +0.2 +0.4 +0.2 -4.5
carbon source 2'-Deoxyinosine 5 mM (C) -0.4 -1.3 -0.5 +0.7 -2.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.5 -0.0 -0.3 -3.6
soil soil sample 8; outgrowth in LB -0.3 -0.5 +0.1 +0.4 -3.2
stress Chloramphenicol 0.015 mg/ml -0.1 +0.5 +0.4 +0.3 -4.6
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -0.0 -0.4 +0.2 +0.4 -3.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.3 +0.4 +0.1 -3.6
stress Chloramphenicol 0.0075 mg/ml -0.0 +0.5 +0.3 +0.1 -3.7
soil soil sample 7; outgrowth in LB -0.0 -0.3 +0.3 +1.1 -3.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.8 +0.3 -0.4 -2.9
carbon source Carbon source D-Mannitol 5 mM -0.0 +0.5 +0.1 +0.7 -3.2
carbon source Putrescine (C) -0.0 +0.5 +0.5 +0.6 -3.1
agar plate interaction control Taped volatile agar plate with no fungus +0.0 +1.0 +0.6 -0.5 -2.6
stress Choline chloride 300 mM +0.1 +0.6 +1.4 +0.3 -3.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +1.2 +0.7 +0.6 -2.6
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