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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_23725 and PS417_23730 are separated by 131 nucleotides
PS417_23730 and PS417_23735 are separated by 330 nucleotides
PS417_23735 and PS417_23740 are separated by 45 nucleotides
PS417_23740 and PS417_23745 overlap by 1 nucleotides
PS417_23725: PS417_23725 - methylmalonate-semialdehyde dehydrogenase, at 5,129,180 to 5,130,697
_23725
PS417_23730: PS417_23730 - LysR family transcriptional regulator, at 5,130,829 to 5,131,776
_23730
PS417_23735: PS417_23735 - hypothetical protein, at 5,132,107 to 5,132,397
_23735
PS417_23740: PS417_23740 - LysR family transcriptional regulator, at 5,132,443 to 5,133,390
_23740
PS417_23745: PS417_23745 - hypothetical protein, at 5,133,390 to 5,134,238
_23745
Group
Condition
PS417
_23725
PS417
_23730
PS417
_23735
PS417
_23740
PS417
_23745
carbon source
L-Valine (C)
-4.9
-4.0
-0.3
+0.2
-0.1
carbon source
L-Valine (C)
-4.0
-3.9
-0.8
-0.0
+0.1
carbon source
L-Leucine (C)
-3.2
-0.4
-0.3
+0.1
-0.0
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
-1.5
+0.1
-0.4
-0.1
+0.1
stress
Gentamicin 0.008 mg/ml
-1.2
-0.1
+0.3
-0.2
-0.1
carbon source
2'-Deoxyinosine 5 mM (C)
-0.9
+0.3
-0.4
+0.1
-0.4
stress
Nalidixic 0.015 mg/ml
-1.4
+0.0
-0.0
+0.0
+0.1
soil
soil sample 8; outgrowth in LB
-1.4
+0.2
+0.1
+0.2
-0.3
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-1.6
+0.2
+0.0
+0.1
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.9
-0.2
+0.2
-0.2
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-1.1
-0.0
-0.3
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.9
+0.4
-0.2
-0.2
+0.0
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.9
-0.1
-0.3
+0.4
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-1.7
+0.3
+0.8
-0.0
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.8
+0.2
+0.5
+0.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-1.1
-0.0
+0.4
+0.1
+0.1
phage
P. simiae Grant Run MOI 10
-1.0
+0.3
+0.4
+0.1
-0.1
soil
soil sample 2; outgrowth in LB
-0.6
+0.4
-0.2
+0.4
-0.1
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.4
+0.2
-0.6
+0.5
+0.3
supernatant control
Vogels_fungal_media 0.1X
+0.9
-0.0
-0.6
+0.1
-0.1
soil
soil sample 5; outgrowth in LB
-1.0
+0.6
+0.1
+0.6
+0.1
carbon source
2-Deoxy-D-Ribose (C)
+0.9
-0.3
-0.1
+0.2
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.8
-0.3
-0.3
+0.4
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.6
+0.3
+0.8
+0.4
+0.2
soil
soil sample 3; outgrowth in LB
+0.7
-0.1
+0.3
+0.4
-0.2
carbon source
Trisodium citrate (C)
-0.2
-0.0
+1.5
+0.2
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.1
-0.2
+0.2
+0.2
+0.3
phage
P. simiae OR Antrim MOI 0.1
+0.9
-0.0
+0.7
-0.0
-0.0
soil
soil sample 7; outgrowth in LB
+0.6
+0.1
+0.4
+0.7
-0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+2.5
+0.3
-0.4
+0.2
-0.1
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