Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_23705 and PS417_23710 overlap by 4 nucleotidesPS417_23710 and PS417_23715 are separated by 385 nucleotidesPS417_23715 and PS417_23720 are separated by 48 nucleotidesPS417_23720 and PS417_23725 are separated by 15 nucleotides PS417_23705: PS417_23705 - Tryptophan 2,3-dioxygenase (EC 1.13.11.11) (from data), at 5,125,994 to 5,126,851 _23705 PS417_23710: PS417_23710 - Kynurenine formamidase, bacterial (EC 3.5.1.9) (from data), at 5,126,848 to 5,127,498 _23710 PS417_23715: PS417_23715 - cupin, at 5,127,884 to 5,128,228 _23715 PS417_23720: PS417_23720 - 3-hydroxyisobutyrate dehydrogenase, at 5,128,277 to 5,129,164 _23720 PS417_23725: PS417_23725 - methylmalonate-semialdehyde dehydrogenase, at 5,129,180 to 5,130,697 _23725
Group Condition PS417_23705 PS417_23710 PS417_23715 PS417_23720 PS417_23725
carbon source L-Tryptophan (C) -4.9 -5.2 +0.1 N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -4.3 -4.2 -1.4 N.D. +1.1
carbon source L-Valine (C) -0.3 -0.3 -0.7 N.D. -4.0
carbon source L-Valine (C) +0.5 -0.3 -0.3 N.D. -4.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 -1.0 -1.5 N.D. -1.8
seeds Growth on radish seeds for 72 hours -0.7 -0.6 -1.4 N.D. -0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.9 -0.7 -1.7 N.D. +0.0
soil soil sample 5; outgrowth in LB -0.8 +0.0 -1.3 N.D. -1.0
seeds Growth on radish seeds for 24 hours -0.4 -0.6 -1.1 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.3 -0.1 -1.0 N.D. -1.1
carbon source L-Leucine (C) +0.0 +0.4 +0.4 N.D. -3.2
phage JP1 MOI 0.1 -0.3 -0.0 -1.4 N.D. -0.6
soil soil sample 2; outgrowth in LB -0.3 -0.5 -0.8 N.D. -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.4 +0.1 -1.0 N.D. +0.3
phage JP1 MOI 10 +0.5 -0.2 -2.0 N.D. +0.2
motility inner cut, LB soft agar motility assay -0.7 -0.6 -0.8 N.D. +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.5 +0.2 N.D. -1.7
phage P. simiae OR1 MOI 0.1 +0.7 -1.0 -0.6 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.8 +0.9 N.D. -0.9
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -0.5 +0.3 +0.8 N.D. -1.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.4 -0.7 +0.5 N.D. +0.7
nophagecontrol Only library +0.7 -0.5 -1.0 N.D. -0.1
soil soil sample 8; outgrowth in LB -0.3 -0.2 +1.2 N.D. -1.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.4 -0.9 N.D. +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.4 -1.5 N.D. +0.8
agar plate interaction control Taped volatile agar plate with no fungus -0.6 +0.7 -0.8 N.D. +0.8
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 -0.4 +1.7 N.D. -0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 +0.2 -0.9 N.D. +2.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 +1.3 +0.5 N.D. +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.5 +1.2 N.D. +0.7
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