Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_23650 and PS417_23655 are separated by 144 nucleotidesPS417_23655 and PS417_23660 are separated by 104 nucleotidesPS417_23660 and PS417_23665 are separated by 41 nucleotidesPS417_23665 and PS417_23670 are separated by 12 nucleotides PS417_23650: PS417_23650 - Kynureninase (EC 3.7.1.3) (from data), at 5,114,613 to 5,115,863 _23650 PS417_23655: PS417_23655 - AsnC family transcriptional regulator, at 5,116,008 to 5,116,481 _23655 PS417_23660: PS417_23660 - Catechol 1,2-dioxygenase (EC 1.13.11.1) (from data), at 5,116,586 to 5,117,515 _23660 PS417_23665: PS417_23665 - Muconolactone isomerase (EC 5.3.3.4) (from data), at 5,117,557 to 5,117,847 _23665 PS417_23670: PS417_23670 - Muconate cycloisomerase (EC 5.5.1.1) (from data), at 5,117,860 to 5,118,987 _23670
Group Condition PS417_23650 PS417_23655 PS417_23660 PS417_23665 PS417_23670
carbon source L-Tryptophan (C) -5.0 -3.9 -3.1 -4.0 -2.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -3.7 -4.0 -0.1 -0.6 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 -0.1 -0.2 -0.6 -0.8
exudates RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x -0.4 -0.2 -0.3 -0.6 -0.5
phage P. simiae ORA MOI 1 -0.2 -0.5 -0.4 -0.3 -0.4
phage P. simiae OR Antrim MOI 10 -0.7 -0.4 -0.2 -0.4 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.0 -0.1 -1.1 -0.5
soil soil sample 3; outgrowth in LB -0.6 +0.3 +0.4 -0.6 -0.8
stress Gentamicin 0.008 mg/ml +0.2 +0.2 +0.0 +0.0 -1.3
supernatant control Vogels_fungal_media 0.1X -0.3 +0.1 +0.1 +0.4 -1.1
phage JP1 MOI 1 -0.1 -0.2 +0.8 -0.9 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.0 -0.8 +0.3 +0.6 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 +0.2 +0.2 +0.1 -1.2
rhizosphere rhizosphere sample 2; outgrowth in LB +0.3 +0.5 +0.1 +0.3 -1.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.7 +0.0 +1.0 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 -0.3 -0.2 +0.7 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.7 -0.0 +0.4 +0.5 -0.3
soil soil sample 5; outgrowth in LB -0.2 -0.2 +0.6 -0.6 +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.7 +0.0 -0.5 +0.7 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.5 -0.2 +0.4 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.0 -0.5 +1.0 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 -0.5 -0.2 +0.5 +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 +0.1 -0.2 +1.0 +0.3
seeds Growth on radish seeds for 24 hours +0.1 +0.4 +0.1 -0.4 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.3 +0.3 -0.1 -0.2 +1.3
seeds Growth on radish seeds for 72 hours +0.4 +0.4 +0.3 -0.4 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.3 +0.2 +0.3 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +1.1 +0.0 +0.4 +0.1
carbon source D-Glucosamine Hydrochloride (C) +2.1 -0.0 -0.0 -0.1 +0.0
carbon source D-Glucosamine Hydrochloride (C) +2.1 +0.0 -0.1 -0.1 +0.3
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