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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_23620 and PS417_23625 are separated by 103 nucleotides
PS417_23625 and PS417_23630 are separated by 30 nucleotides
PS417_23630 and PS417_23635 are separated by 120 nucleotides
PS417_23635 and PS417_23640 are separated by 123 nucleotides
PS417_23620: PS417_23620 - epimerase, at 5,108,711 to 5,109,604
_23620
PS417_23625: PS417_23625 - LysR family transcriptional regulator, at 5,109,708 to 5,110,604
_23625
PS417_23630: PS417_23630 - NADPH quinone reductase MdaB, at 5,110,635 to 5,111,225
_23630
PS417_23635: PS417_23635 - membrane protein, at 5,111,346 to 5,112,110
_23635
PS417_23640: PS417_23640 - LysR family transcriptional regulator, at 5,112,234 to 5,113,124
_23640
Group
Condition
PS417
_23620
PS417
_23625
PS417
_23630
PS417
_23635
PS417
_23640
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.2
-1.4
-0.5
-0.1
-0.4
phage
P. simiae OR1 MOI 0.1
-0.8
-0.9
-0.1
+0.1
-0.7
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.7
-1.1
+0.0
-0.6
+0.3
phage
JP1 MOI 1
-0.1
-2.0
+0.6
+0.4
-0.3
rhizosphere
rhizosphere sample 8; outgrowth in LB
-0.3
-2.5
+0.4
+1.0
+0.1
soil
soil sample 7; outgrowth in LB
-0.2
-1.4
+0.4
+0.3
-0.3
phage
JP1 MOI 0.1
+0.4
-0.4
-0.5
-0.3
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.6
-0.6
+0.2
+0.3
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
-1.7
+0.5
+0.3
-0.2
soil
soil sample 5; outgrowth in LB
-0.7
-0.6
-0.3
+0.7
-0.1
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
-0.4
-1.0
+0.3
+0.4
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-1.3
+0.3
+0.3
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.4
-0.5
+0.4
+0.4
-0.5
seeds
Growth on radish seeds for 72 hours
+0.9
-0.4
-0.0
-0.2
-0.7
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
-0.6
+0.0
-0.1
+0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.7
+0.6
-0.3
+0.2
phage
P. simiae ORA MOI 10
+0.6
-0.6
-0.3
-0.2
+0.4
phage
Wcs_0.1
+0.6
-0.5
-0.3
-0.1
+0.6
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.0
+1.0
+0.1
-0.8
+0.1
carbon source
L-Valine (C)
+0.3
-1.0
+0.0
+0.1
+0.9
phage
P. simiae ORA MOI 0.1
+0.6
+0.3
-0.0
+0.7
-0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.0
-0.5
-0.2
+1.2
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.2
+0.7
-0.3
-0.3
+0.5
soil
soil sample 8; outgrowth in LB
-0.4
+0.6
-0.0
+0.9
-0.1
root
root sample 6; outgrowth in LB
-0.0
-0.7
+0.5
+1.7
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
+1.5
-0.1
+0.3
+0.2
rhizosphere
rhizosphere sample 1; outgrowth in LB
+0.0
-0.2
+0.2
+1.9
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
+1.4
+0.2
-0.1
-0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.0
+0.8
+0.3
+0.2
+0.7
ecofab
LB in EcoFAB_3.5mL
+0.6
+1.5
-0.0
+0.1
+0.3
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