Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_02335 and PS417_02340 are separated by 101 nucleotidesPS417_02340 and PS417_02345 are separated by 23 nucleotidesPS417_02345 and PS417_02350 are separated by 120 nucleotidesPS417_02350 and PS417_02355 are separated by 462 nucleotides PS417_02335: PS417_02335 - multidrug DMT transporter, at 533,727 to 534,062 _02335 PS417_02340: PS417_02340 - LysR family transcriptional regulator, at 534,164 to 535,054 _02340 PS417_02345: PS417_02345 - 3-deoxy-D-manno-octulosonic acid transferase, at 535,078 to 536,355 _02345 PS417_02350: PS417_02350 - channel protein TolC, at 536,476 to 537,915 _02350 PS417_02355: PS417_02355 - thiamine biosynthesis protein ThiC, at 538,378 to 540,270 _02355
Group Condition PS417_02335 PS417_02340 PS417_02345 PS417_02350 PS417_02355
stress 1-ethyl-3-methylimidazolium chloride 200 mM -2.6 -2.1 N.D. +0.1 -0.1
stress Choline Lysine 25 mM -2.3 -1.9 N.D. +0.1 -0.2
stress methylglyoxal 0.032 vol% -0.9 -0.4 N.D. -0.6 -0.4
stress Benzalkonium Chloride 0.012 mg/ml -0.9 -1.0 N.D. -0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.1 N.D. -0.6 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 +0.3 N.D. -0.3 -0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.8 -0.8 N.D. -0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.8 N.D. +0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.6 -0.7 N.D. +0.2 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.5 -1.0 N.D. -0.0 -0.3
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -1.0 -0.3 N.D. +0.1 +0.3
phage P. simiae OR Antrim MOI 1 -1.1 -0.1 N.D. +0.3 +0.1
carbon source L-Valine (C) +0.5 -1.6 N.D. +0.2 +0.3
solid stress Fraxetin 0.5 mM; solid stress -0.6 +0.6 N.D. +0.0 -0.5
phage JP1 MOI 1 -0.3 -1.0 N.D. +1.1 -0.3
phage JP1 MOI 10 +0.8 -1.0 N.D. +0.4 -0.5
stress 1-ethyl-3-methylimidazolium acetate 35 mM -2.6 +2.7 N.D. -0.3 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.3 +0.5 N.D. +0.3 -0.9
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.1 -0.4 N.D. -0.3 +0.1
solid stress 80% Methanol 3 mM; solid stress -0.2 +0.9 N.D. +0.3 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.4 -0.7 N.D. -0.2 +0.1
phage P. simiae ORA MOI 0.1 +0.7 +0.7 N.D. -0.7 +0.0
carbon source 2'-Deoxyinosine 5 mM (C) -0.2 +0.7 N.D. +0.5 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +0.8 N.D. -0.2 +0.0
motility inner cut, LB soft agar motility assay +0.4 +0.3 N.D. +0.8 -0.1
motility inner cut, LB soft agar motility assay +0.6 +0.5 N.D. +0.6 -0.2
phage JP1 MOI 0.1 +0.2 +0.7 N.D. +0.7 +0.2
seeds Growth on radish seeds for 72 hours +0.5 +1.3 N.D. +0.4 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.3 -0.0 N.D. +0.8 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.9 +0.7 N.D. +0.1 +0.4
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