Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_23155 and PS417_23160 are separated by 12 nucleotidesPS417_23160 and PS417_23165 are separated by 29 nucleotidesPS417_23165 and PS417_23170 are separated by 92 nucleotidesPS417_23170 and PS417_23175 are separated by 14 nucleotides PS417_23155: PS417_23155 - pilus assembly protein PilW, at 5,019,500 to 5,020,258 _23155 PS417_23160: PS417_23160 - 50S rRNA methyltransferase, at 5,020,271 to 5,021,419 _23160 PS417_23165: PS417_23165 - nucleoside diphosphate kinase, at 5,021,449 to 5,021,874 _23165 PS417_23170: PS417_23170 - FeS assembly protein IscX, at 5,021,967 to 5,022,167 _23170 PS417_23175: PS417_23175 - 2Fe-2S ferredoxin, at 5,022,182 to 5,022,523 _23175
Group Condition PS417_23155 PS417_23160 PS417_23165 PS417_23170 PS417_23175
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -1.9 N.D. -1.1 N.D.
nitrogen source Uridine (N) +0.0 -0.3 N.D. -2.9 N.D.
agar plate interaction control Taped volatile agar plate with no fungus -0.5 -1.2 N.D. -1.5 N.D.
stress Doxycycline hyclate 0.001 mg/ml -1.1 +0.4 N.D. -1.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 -0.5 N.D. -1.4 N.D.
solid stress Fraxetin 3 mM; solid stress -0.8 -1.1 N.D. -0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 +0.2 N.D. -2.6 N.D.
phage JP1 MOI 10 +0.0 -1.0 N.D. -1.1 N.D.
stress Gentamicin 0.008 mg/ml -1.9 +1.0 N.D. -1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.7 +1.0 N.D. -3.7 N.D.
phage P. simiae ORA MOI 10 +0.7 -0.6 N.D. -1.6 N.D.
soil soil sample 6; outgrowth in LB +0.6 -0.7 N.D. -1.0 N.D.
motility inner cut, LB soft agar motility assay +0.5 -0.8 N.D. -0.9 N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB +0.7 -0.6 N.D. -1.1 N.D.
carbon source 2'-Deoxyinosine 5 mM (C) +0.5 +0.9 N.D. -2.3 N.D.
motility inner cut, LB soft agar motility assay +0.4 -1.6 N.D. +0.4 N.D.
supernatant control Vogels_fungal_media 0.1X -0.3 -1.2 N.D. +0.7 N.D.
motility outer cut, LB soft agar motility assay +0.2 -1.5 N.D. +0.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -1.3 N.D. +0.4 N.D.
phage P. simiae Grant Run MOI 0.1 +0.9 -0.6 N.D. -0.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -1.0 N.D. +1.0 N.D.
phage P. simiae OR1 MOI 0.1 +1.3 +0.0 N.D. -0.9 N.D.
ecofab LB in EcoFAB_3.5mL +1.2 -0.8 N.D. +0.4 N.D.
lb LB_control +0.8 -0.7 N.D. +0.7 N.D.
nophagecontrol Only library +0.9 -1.0 N.D. +1.0 N.D.
phage P. simiae Grant Run MOI 0.1 +0.9 -0.6 N.D. +0.6 N.D.
seeds Growth on radish seeds for 24 hours +1.1 +0.1 N.D. +0.9 N.D.
seeds Growth on radish seeds for 72 hours +0.8 +0.8 N.D. +0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.6 -0.5 N.D. +0.5 N.D.
seeds Growth on radish seeds for 72 hours +1.2 +0.2 N.D. +1.8 N.D.
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