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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_23130 and PS417_23135 overlap by 8 nucleotides
PS417_23135 and PS417_23140 are separated by 41 nucleotides
PS417_23140 and PS417_23145 are separated by 21 nucleotides
PS417_23145 and PS417_23150 are separated by 2 nucleotides
PS417_23130: PS417_23130 - dehydrogenase, at 5,014,267 to 5,015,418
_23130
PS417_23135: PS417_23135 - GTP-binding protein, at 5,015,411 to 5,016,052
_23135
PS417_23140: PS417_23140 - histidinol dehydrogenase, at 5,016,094 to 5,017,383
_23140
PS417_23145: PS417_23145 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 5,017,405 to 5,018,514
_23145
PS417_23150: PS417_23150 - Cro/Cl family transcriptional regulator, at 5,018,517 to 5,019,500
_23150
Group
Condition
PS417
_23130
PS417
_23135
PS417
_23140
PS417
_23145
PS417
_23150
stress
Doxycycline hyclate 0.001 mg/ml
N.D.
-1.9
N.D.
N.D.
-1.5
stress
Gentamicin 0.008 mg/ml
N.D.
-1.6
N.D.
N.D.
-1.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-2.9
N.D.
N.D.
+0.0
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
-1.3
N.D.
N.D.
-1.2
stress
Spectinomycin 0.4 mg/ml
N.D.
-1.2
N.D.
N.D.
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.5
N.D.
N.D.
-1.8
rhizosphere
rhizosphere sample 2; outgrowth in LB
N.D.
+0.5
N.D.
N.D.
-1.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
-1.8
N.D.
N.D.
+0.7
supernatant control
Vogels_fungal_media 0.1X
N.D.
-1.5
N.D.
N.D.
+0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-1.4
N.D.
N.D.
+0.8
liquid stress
Fraxetin 3 mM; liquid stress
N.D.
-1.4
N.D.
N.D.
+1.0
solid stress
Fraxetin 3 mM; solid stress
N.D.
-1.2
N.D.
N.D.
+1.3
solid stress
Fraxetin 3 mM; solid stress
N.D.
-1.1
N.D.
N.D.
+1.6
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
+0.5
N.D.
N.D.
+1.5
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
+0.6
N.D.
N.D.
+1.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+1.6
N.D.
N.D.
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+1.0
N.D.
N.D.
+1.1
phage
P. simiae OR Antrim MOI 0.1
N.D.
+1.2
N.D.
N.D.
+1.0
phage
P. simiae OR Antrim MOI 0.1
N.D.
+1.0
N.D.
N.D.
+1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.7
N.D.
N.D.
+1.5
phage
JP1 MOI 1
N.D.
+1.2
N.D.
N.D.
+1.1
stress
methylglyoxal 0.032 vol%
N.D.
+1.2
N.D.
N.D.
+1.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+1.9
N.D.
N.D.
+0.4
phage
P. simiae OR1 MOI 0.1
N.D.
+0.8
N.D.
N.D.
+1.5
phage
P. simiae Grant Run MOI 0.1
N.D.
+1.1
N.D.
N.D.
+1.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+1.0
N.D.
N.D.
+1.4
nophagecontrol
Only library
N.D.
+1.3
N.D.
N.D.
+1.1
phage
P. simiae Grant Run MOI 0.1
N.D.
+1.5
N.D.
N.D.
+1.3
phage
JP1 MOI 0.1
N.D.
+2.5
N.D.
N.D.
+0.8
stress
1-ethyl-3-methylimidazolium chloride 200 mM
N.D.
+1.2
N.D.
N.D.
+2.7
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