Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_23055 and PS417_23060 are separated by 13 nucleotidesPS417_23060 and PS417_23065 are separated by 167 nucleotidesPS417_23065 and PS417_23070 are separated by 340 nucleotidesPS417_23070 and PS417_23075 are separated by 80 nucleotides PS417_23055: PS417_23055 - GntR family transcriptional regulator, at 4,997,949 to 4,998,653 _23055 PS417_23060: PS417_23060 - aminoglycoside phosphotransferase, at 4,998,667 to 4,999,437 _23060 PS417_23065: PS417_23065 - hypothetical protein, at 4,999,605 to 5,000,162 _23065 PS417_23070: PS417_23070 - GMP synthase, at 5,000,503 to 5,002,080 _23070 PS417_23075: PS417_23075 - inosine-5-monophosphate dehydrogenase, at 5,002,161 to 5,003,630 _23075
Group Condition PS417_23055 PS417_23060 PS417_23065 PS417_23070 PS417_23075
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.8 -0.2 N.D. N.D.
phage JP1 MOI 10 N.D. -0.6 -0.3 N.D. N.D.
phage JP1 MOI 10 N.D. -0.5 -0.4 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.6 -0.1 N.D. N.D.
phage P. simiae Grant Run MOI 10 N.D. -0.5 -0.1 N.D. N.D.
motility outer cut, LB soft agar motility assay N.D. -0.6 +0.1 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -0.5 +0.2 N.D. N.D.
motility outer cut, LB soft agar motility assay N.D. +0.3 -0.6 N.D. N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x N.D. -0.5 +0.4 N.D. N.D.
soil soil sample 8; outgrowth in LB N.D. -0.4 +0.3 N.D. N.D.
liquid stress 80% Methanol 3 mM; liquid stress N.D. -0.4 +0.3 N.D. N.D.
carbon source 2'-Deoxyinosine 5 mM (C) N.D. +0.4 -0.3 N.D. N.D.
carbon source Glycerol (C) N.D. +0.4 -0.2 N.D. N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x N.D. -0.2 +0.5 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 -0.2 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.9 -0.4 N.D. N.D.
liquid stress 80% Methanol 2 mM; liquid stress N.D. +0.4 +0.2 N.D. N.D.
no stress control on plate growth on LB_noSalt plate N.D. +0.4 +0.2 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 +0.3 N.D. N.D.
soil soil sample 4; outgrowth in LB N.D. +0.7 -0.0 N.D. N.D.
soil soil sample 5; outgrowth in LB N.D. +0.7 -0.1 N.D. N.D.
r2a control with 0.2x vogels R2A control with 0.2X Vogels N.D. +0.6 +0.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.4 +0.3 N.D. N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 +0.4 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.3 +0.4 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.4 +0.4 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.8 +0.1 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.9 +0.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +1.0 +0.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +1.5 +0.0 N.D. N.D.
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