Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_02225 and PS417_02230 are separated by 54 nucleotidesPS417_02230 and PS417_02235 are separated by 1 nucleotidesPS417_02235 and PS417_02240 overlap by 1 nucleotidesPS417_02240 and PS417_02245 overlap by 4 nucleotides PS417_02225: PS417_02225 - bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase, at 505,356 to 508,295 _02225 PS417_02230: PS417_02230 - ADP-heptose--LPS heptosyltransferase, at 508,350 to 509,384 _02230 PS417_02235: PS417_02235 - ADP-heptose--LPS heptosyltransferase, at 509,386 to 510,447 _02235 PS417_02240: PS417_02240 - glucosyltransferase I RfaG, at 510,447 to 511,571 _02240 PS417_02245: PS417_02245 - heptose kinase, at 511,568 to 512,374 _02245
Group Condition PS417_02225 PS417_02230 PS417_02235 PS417_02240 PS417_02245
solid stress Fraxetin 2 mM; solid stress -4.8 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -4.8 N.D. N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -4.6 N.D. N.D. N.D. N.D.
solid stress Fraxetin 3 mM; solid stress -4.6 N.D. N.D. N.D. N.D.
liquid stress Fraxetin 3 mM; liquid stress -3.3 N.D. N.D. N.D. N.D.
solid stress Fraxetin 3 mM; solid stress -3.0 N.D. N.D. N.D. N.D.
carbon source Putrescine (C) -2.8 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -2.7 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.7 N.D. N.D. N.D. N.D.
phage P. simiae OR1 MOI 0.1 -2.7 N.D. N.D. N.D. N.D.
agar plate interaction control Taped volatile agar plate with no fungus -2.6 N.D. N.D. N.D. N.D.
phage P. simiae Grant Run MOI 10 -2.6 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.6 N.D. N.D. N.D. N.D.
soil soil sample 2; outgrowth in LB -2.5 N.D. N.D. N.D. N.D.
phage P. simiae ORA MOI 0.1 -2.4 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.4 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.3 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -2.3 N.D. N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -2.3 N.D. N.D. N.D. N.D.
seeds Growth on radish seeds for 72 hours -2.2 N.D. N.D. N.D. N.D.
phage P. simiae Grant Run MOI 0.1 -2.2 N.D. N.D. N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.2 N.D. N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay -2.1 N.D. N.D. N.D. N.D.
stress LB_noSalt with Chloride 1100 mM -2.0 N.D. N.D. N.D. N.D.
liquid stress Fraxetin 3 mM; liquid stress -2.0 N.D. N.D. N.D. N.D.
solid stress Fraxetin 3 mM; solid stress -2.0 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -2.0 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.0 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +2.5 N.D. N.D. N.D. N.D.
stress Gentamicin 0.008 mg/ml +2.5 N.D. N.D. N.D. N.D.
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