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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_22295 and PS417_22300 are separated by 263 nucleotides
PS417_22300 and PS417_22305 are separated by 68 nucleotides
PS417_22305 and PS417_22310 are separated by 37 nucleotides
PS417_22310 and PS417_22315 are separated by 114 nucleotides
PS417_22295: PS417_22295 - chemotaxis protein CheY, at 4,828,736 to 4,829,413
_22295
PS417_22300: PS417_22300 - LTXXQ domain protein, at 4,829,677 to 4,830,141
_22300
PS417_22305: PS417_22305 - histidine kinase, at 4,830,210 to 4,831,553
_22305
PS417_22310: PS417_22310 - CAIB/BAIF family CoA transferase, at 4,831,591 to 4,832,940
_22310
PS417_22315: PS417_22315 - DNA-3-methyladenine glycosylase, at 4,833,055 to 4,833,918
_22315
Group
Condition
PS417
_22295
PS417
_22300
PS417
_22305
PS417
_22310
PS417
_22315
phage
JP1 MOI 10
N.D.
-1.9
-0.9
-0.1
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.3
-0.2
+0.3
-1.5
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
N.D.
-1.8
+0.0
-0.1
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-0.5
-0.2
-0.2
-1.3
supernatant control
Vogels_fungal_media 0.1X
N.D.
-0.5
-0.3
-0.8
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-1.1
-0.9
-0.2
+0.4
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
+0.4
-1.5
-0.2
-0.1
nophagecontrol
Only library
N.D.
-1.4
-0.1
-0.5
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
-1.2
+0.3
-0.0
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.8
+0.1
+0.3
-0.9
supernatant
RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
N.D.
-1.5
+0.2
-0.2
+0.3
soil
soil sample 6; outgrowth in LB
N.D.
-0.6
-0.8
+0.4
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.8
-0.8
+0.3
+0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+1.2
+0.2
-0.4
-1.8
soil
soil sample 7; outgrowth in LB
N.D.
+0.4
+0.1
+0.2
-1.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
-0.4
-0.7
-0.3
+0.8
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-1.5
-0.4
+0.3
+1.2
rhizosphere
rhizosphere sample 8; outgrowth in LB
N.D.
-0.5
-0.2
+1.0
-0.5
rhizosphere
rhizosphere sample 1; outgrowth in LB
N.D.
-0.4
-0.3
+1.8
-0.5
root
root sample 6; outgrowth in LB
N.D.
-0.1
-1.3
+1.3
+0.6
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
N.D.
-0.7
+0.5
+0.4
+0.4
soil
soil sample 5; outgrowth in LB
N.D.
-0.3
-0.5
+1.2
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
-0.8
+0.7
+0.5
+0.2
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
+0.6
-1.0
+0.8
+0.4
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
+1.0
+0.5
-0.0
-0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
-0.8
+0.3
+0.1
+1.6
phage
JP1 MOI 1
N.D.
+0.9
+0.6
+0.3
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+1.2
-0.3
+0.1
+0.6
seeds
Growth on radish seeds for 72 hours
N.D.
+1.7
+0.6
-0.1
-0.4
carbon source
L-Valine (C)
N.D.
+1.0
-0.4
-0.1
+1.4
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