Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_22295 and PS417_22300 are separated by 263 nucleotidesPS417_22300 and PS417_22305 are separated by 68 nucleotidesPS417_22305 and PS417_22310 are separated by 37 nucleotidesPS417_22310 and PS417_22315 are separated by 114 nucleotides PS417_22295: PS417_22295 - chemotaxis protein CheY, at 4,828,736 to 4,829,413 _22295 PS417_22300: PS417_22300 - LTXXQ domain protein, at 4,829,677 to 4,830,141 _22300 PS417_22305: PS417_22305 - histidine kinase, at 4,830,210 to 4,831,553 _22305 PS417_22310: PS417_22310 - CAIB/BAIF family CoA transferase, at 4,831,591 to 4,832,940 _22310 PS417_22315: PS417_22315 - DNA-3-methyladenine glycosylase, at 4,833,055 to 4,833,918 _22315
Group Condition PS417_22295 PS417_22300 PS417_22305 PS417_22310 PS417_22315
phage JP1 MOI 10 N.D. -1.9 -0.9 -0.1 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.3 -0.2 +0.3 -1.5
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. -1.8 +0.0 -0.1 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.5 -0.2 -0.2 -1.3
supernatant control Vogels_fungal_media 0.1X N.D. -0.5 -0.3 -0.8 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.1 -0.9 -0.2 +0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +0.4 -1.5 -0.2 -0.1
nophagecontrol Only library N.D. -1.4 -0.1 -0.5 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -1.2 +0.3 -0.0 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.8 +0.1 +0.3 -0.9
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. -1.5 +0.2 -0.2 +0.3
soil soil sample 6; outgrowth in LB N.D. -0.6 -0.8 +0.4 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.8 -0.8 +0.3 +0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +1.2 +0.2 -0.4 -1.8
soil soil sample 7; outgrowth in LB N.D. +0.4 +0.1 +0.2 -1.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.4 -0.7 -0.3 +0.8
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.5 -0.4 +0.3 +1.2
rhizosphere rhizosphere sample 8; outgrowth in LB N.D. -0.5 -0.2 +1.0 -0.5
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. -0.4 -0.3 +1.8 -0.5
root root sample 6; outgrowth in LB N.D. -0.1 -1.3 +1.3 +0.6
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. -0.7 +0.5 +0.4 +0.4
soil soil sample 5; outgrowth in LB N.D. -0.3 -0.5 +1.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.8 +0.7 +0.5 +0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.6 -1.0 +0.8 +0.4
agar plate interaction control Taped volatile agar plate with no fungus N.D. +1.0 +0.5 -0.0 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.8 +0.3 +0.1 +1.6
phage JP1 MOI 1 N.D. +0.9 +0.6 +0.3 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +1.2 -0.3 +0.1 +0.6
seeds Growth on radish seeds for 72 hours N.D. +1.7 +0.6 -0.1 -0.4
carbon source L-Valine (C) N.D. +1.0 -0.4 -0.1 +1.4
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