Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_22245 and PS417_22250 are separated by 196 nucleotidesPS417_22250 and PS417_22255 are separated by 144 nucleotidesPS417_22255 and PS417_22260 are separated by 56 nucleotidesPS417_22260 and PS417_22265 are separated by 12 nucleotides PS417_22245: PS417_22245 - leucyl aminopeptidase (aminopeptidase T), at 4,821,193 to 4,822,152 _22245 PS417_22250: PS417_22250 - pseudouridine synthase, at 4,822,349 to 4,823,560 _22250 PS417_22255: PS417_22255 - Fe-S oxidoreductase, at 4,823,705 to 4,823,896 _22255 PS417_22260: PS417_22260 - segregation and condensation protein B, at 4,823,953 to 4,824,891 _22260 PS417_22265: PS417_22265 - chromosome segregation protein ScpA, at 4,824,904 to 4,825,722 _22265
Group Condition PS417_22245 PS417_22250 PS417_22255 PS417_22260 PS417_22265
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.4 -0.6 N.D. -1.2 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 -1.3 N.D. -0.7 -0.3
stress LB_noSalt with Chloride 1100 mM +0.0 -1.9 N.D. -0.2 -0.2
phage P. simiae ORA MOI 1 -0.7 -0.8 N.D. -0.4 -0.2
soil soil sample 4; outgrowth in LB -0.3 -1.0 N.D. -0.3 -0.4
agar plate interaction control Taped volatile agar plate with no fungus -0.6 -0.1 N.D. -0.9 -0.3
motility inner cut, LB soft agar motility assay +0.2 -0.5 N.D. -1.4 -0.2
phage JP1 MOI 0.1 +0.1 -1.5 N.D. +0.2 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -1.2 N.D. -0.4 -0.1
phage P. simiae Grant Run MOI 0.1 -0.1 -1.0 N.D. -0.7 -0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 -0.9 N.D. -0.4 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.3 N.D. -1.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.1 N.D. -1.6 -0.1
phage JP1 MOI 10 -0.5 -0.5 N.D. -0.7 -0.1
motility inner cut, LB soft agar motility assay +0.1 -0.1 N.D. -1.7 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -1.2 N.D. -0.4 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.7 N.D. -1.4 +0.2
motility outer cut, LB soft agar motility assay +0.1 -0.4 N.D. -1.1 -0.2
phage P. simiae OR Antrim MOI 0.1 -0.3 -0.6 N.D. +0.4 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.9 N.D. -0.5 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.4 N.D. -1.1 -0.0
carbon source L-Valine (C) -0.2 +0.7 N.D. -0.2 -0.9
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 -1.4 N.D. +0.8 +0.1
phage P. simiae OR Antrim MOI 10 +0.2 -0.9 N.D. +0.6 -0.4
carbon source L-Valine (C) -0.8 +0.7 N.D. -0.2 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 +0.6 N.D. -1.4 +0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 -0.1 N.D. -0.7 +0.2
carbon source 2'-Deoxyinosine 5 mM (C) -0.1 +0.4 N.D. -0.7 +0.7
stress methylglyoxal 0.032 vol% -0.2 -0.2 N.D. +1.5 +0.4
stress Gentamicin 0.008 mg/ml +0.7 +1.7 N.D. -0.5 +0.4
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