Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_22085 and PS417_22090 are separated by 205 nucleotidesPS417_22090 and PS417_22095 are separated by 12 nucleotidesPS417_22095 and PS417_22100 are separated by 93 nucleotidesPS417_22100 and PS417_22105 are separated by 17 nucleotides PS417_22085: PS417_22085 - aldehyde dehydrogenase, at 4,785,834 to 4,786,301 _22085 PS417_22090: PS417_22090 - hypothetical protein, at 4,786,507 to 4,786,743 _22090 PS417_22095: PS417_22095 - class V aminotransferase, at 4,786,756 to 4,787,931 _22095 PS417_22100: PS417_22100 - keto-deoxy-phosphogluconate aldolase, at 4,788,025 to 4,788,690 _22100 PS417_22105: PS417_22105 - 6-phosphogluconolactonase, at 4,788,708 to 4,789,421 _22105
Group Condition PS417_22085 PS417_22090 PS417_22095 PS417_22100 PS417_22105
phage JP1 MOI 0.1 -1.6 N.D. -0.4 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.8 N.D. -0.0 N.D. N.D.
nophagecontrol Only library -1.3 N.D. -0.1 N.D. N.D.
seeds Growth on radish seeds for 72 hours -0.8 N.D. -0.3 N.D. N.D.
seeds Growth on radish seeds for 72 hours -0.7 N.D. -0.5 N.D. N.D.
no phage control no phage control -0.8 N.D. -0.3 N.D. N.D.
seeds Growth on radish seeds for 72 hours -1.3 N.D. +0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 N.D. -0.3 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.1 N.D. +0.1 N.D. N.D.
solid stress Scopoletin 3 mM; solid stress -1.1 N.D. +0.1 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.4 N.D. +0.5 N.D. N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x -0.9 N.D. +0.1 N.D. N.D.
stress Gentamicin 0.008 mg/ml -1.2 N.D. +0.3 N.D. N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x -0.9 N.D. +0.2 N.D. N.D.
carbon source 2'-Deoxyinosine 5 mM (C) -0.9 N.D. +0.3 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 N.D. -0.4 N.D. N.D.
phage P. simiae Grant Run MOI 10 +0.8 N.D. -0.3 N.D. N.D.
stress Neomycin 0.04 mg/ml +0.9 N.D. -0.2 N.D. N.D.
carbon source L-Valine (C) +1.1 N.D. -0.1 N.D. N.D.
phage P. simiae ORA MOI 0.1 +0.9 N.D. +0.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 N.D. +0.2 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.6 N.D. +0.5 N.D. N.D.
phage JP1 MOI 10 +1.1 N.D. +0.0 N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.8 N.D. +0.3 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 N.D. +0.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.0 N.D. +0.3 N.D. N.D.
phage JP1 MOI 1 +1.2 N.D. +0.2 N.D. N.D.
stress methylglyoxal 0.032 vol% +2.3 N.D. -0.9 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 N.D. +0.8 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.5 N.D. +0.3 N.D. N.D.
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