Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_22065 and PS417_22070 are separated by 395 nucleotidesPS417_22070 and PS417_22075 are separated by 210 nucleotidesPS417_22075 and PS417_22080 are separated by 57 nucleotidesPS417_22080 and PS417_22085 are separated by 55 nucleotides PS417_22065: PS417_22065 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) (from data), at 4,780,320 to 4,782,011 _22065 PS417_22070: PS417_22070 - long-chain fatty acid--CoA ligase, at 4,782,407 to 4,784,095 _22070 PS417_22075: PS417_22075 - alpha/beta hydrolase, at 4,784,306 to 4,785,250 _22075 PS417_22080: PS417_22080 - 3-hydroxybutyryl-CoA dehydratase, at 4,785,308 to 4,785,778 _22080 PS417_22085: PS417_22085 - aldehyde dehydrogenase, at 4,785,834 to 4,786,301 _22085
Group Condition PS417_22065 PS417_22070 PS417_22075 PS417_22080 PS417_22085
carbon source octanoate (C) -5.6 -0.3 +0.6 N.D. -0.5
carbon source octanoate (C) -5.4 -0.3 +0.7 N.D. -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.2 -0.6 N.D. -1.8
solid stress Fraxetin 3 mM; solid stress -1.7 -0.3 -0.0 N.D. -0.3
seeds Growth on radish seeds for 72 hours +0.1 -0.1 -0.4 N.D. -1.3
nophagecontrol Only library +0.2 -0.3 -0.0 N.D. -1.3
liquid stress Fraxetin 3 mM; liquid stress -1.7 +0.1 +0.1 N.D. +0.1
phage JP1 MOI 0.1 +0.5 -0.1 +0.4 N.D. -1.6
solid stress Fraxetin 3 mM; solid stress -1.1 -0.1 +0.0 N.D. +0.4
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x +0.4 -0.3 +0.1 N.D. -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.2 -0.4 N.D. -0.9
stress Gentamicin 0.008 mg/ml +0.7 -0.0 +0.0 N.D. -1.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +0.6 -0.5 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.2 +0.9 N.D. -1.4
phage P. simiae OR1 MOI 0.1 +0.7 +0.2 +0.0 N.D. -0.8
phage P. simiae ORA MOI 1 +1.0 -0.5 -0.1 N.D. +0.1
stress Doxycycline hyclate 0.001 mg/ml +1.5 -0.1 -0.4 N.D. -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.5 -0.1 N.D. +0.8
phage JP1 MOI 10 +1.1 +0.3 -0.2 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.5 -0.4 N.D. +0.5
phage P. simiae Grant Run MOI 10 +0.9 -0.3 -0.3 N.D. +0.8
carbon source L-Valine (C) -0.2 -0.1 +0.5 N.D. +1.1
phage P. simiae OR Antrim MOI 1 +0.6 +0.2 -0.3 N.D. +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.3 +0.1 +0.3 N.D. +0.9
phage P. simiae ORA MOI 0.1 +0.5 +0.1 +0.1 N.D. +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +0.3 -0.1 N.D. +0.9
phage JP1 MOI 1 +0.7 +0.1 -0.1 N.D. +1.2
phage JP1 MOI 10 +1.1 -0.1 +0.1 N.D. +1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 +0.3 -0.2 N.D. +1.5
stress methylglyoxal 0.032 vol% +0.6 -0.5 +0.3 N.D. +2.3
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