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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_22065 and PS417_22070 are separated by 395 nucleotides
PS417_22070 and PS417_22075 are separated by 210 nucleotides
PS417_22075 and PS417_22080 are separated by 57 nucleotides
PS417_22080 and PS417_22085 are separated by 55 nucleotides
PS417_22065: PS417_22065 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
(from data)
, at 4,780,320 to 4,782,011
_22065
PS417_22070: PS417_22070 - long-chain fatty acid--CoA ligase, at 4,782,407 to 4,784,095
_22070
PS417_22075: PS417_22075 - alpha/beta hydrolase, at 4,784,306 to 4,785,250
_22075
PS417_22080: PS417_22080 - 3-hydroxybutyryl-CoA dehydratase, at 4,785,308 to 4,785,778
_22080
PS417_22085: PS417_22085 - aldehyde dehydrogenase, at 4,785,834 to 4,786,301
_22085
Group
Condition
PS417
_22065
PS417
_22070
PS417
_22075
PS417
_22080
PS417
_22085
carbon source
octanoate (C)
-5.6
-0.3
+0.6
N.D.
-0.5
carbon source
octanoate (C)
-5.4
-0.3
+0.7
N.D.
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.0
-0.2
-0.6
N.D.
-1.8
solid stress
Fraxetin 3 mM; solid stress
-1.7
-0.3
-0.0
N.D.
-0.3
seeds
Growth on radish seeds for 72 hours
+0.1
-0.1
-0.4
N.D.
-1.3
nophagecontrol
Only library
+0.2
-0.3
-0.0
N.D.
-1.3
liquid stress
Fraxetin 3 mM; liquid stress
-1.7
+0.1
+0.1
N.D.
+0.1
phage
JP1 MOI 0.1
+0.5
-0.1
+0.4
N.D.
-1.6
solid stress
Fraxetin 3 mM; solid stress
-1.1
-0.1
+0.0
N.D.
+0.4
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
+0.4
-0.3
+0.1
N.D.
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
+0.2
-0.4
N.D.
-0.9
stress
Gentamicin 0.008 mg/ml
+0.7
-0.0
+0.0
N.D.
-1.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.0
+0.6
-0.5
N.D.
-0.6
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.2
+0.9
N.D.
-1.4
phage
P. simiae OR1 MOI 0.1
+0.7
+0.2
+0.0
N.D.
-0.8
phage
P. simiae ORA MOI 1
+1.0
-0.5
-0.1
N.D.
+0.1
stress
Doxycycline hyclate 0.001 mg/ml
+1.5
-0.1
-0.4
N.D.
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.5
-0.1
N.D.
+0.8
phage
JP1 MOI 10
+1.1
+0.3
-0.2
N.D.
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
+0.5
-0.4
N.D.
+0.5
phage
P. simiae Grant Run MOI 10
+0.9
-0.3
-0.3
N.D.
+0.8
carbon source
L-Valine (C)
-0.2
-0.1
+0.5
N.D.
+1.1
phage
P. simiae OR Antrim MOI 1
+0.6
+0.2
-0.3
N.D.
+0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.3
+0.1
+0.3
N.D.
+0.9
phage
P. simiae ORA MOI 0.1
+0.5
+0.1
+0.1
N.D.
+0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
+0.3
-0.1
N.D.
+0.9
phage
JP1 MOI 1
+0.7
+0.1
-0.1
N.D.
+1.2
phage
JP1 MOI 10
+1.1
-0.1
+0.1
N.D.
+1.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.9
+0.3
-0.2
N.D.
+1.5
stress
methylglyoxal 0.032 vol%
+0.6
-0.5
+0.3
N.D.
+2.3
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