Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_22060 and PS417_22065 are separated by 114 nucleotidesPS417_22065 and PS417_22070 are separated by 395 nucleotidesPS417_22070 and PS417_22075 are separated by 210 nucleotidesPS417_22075 and PS417_22080 are separated by 57 nucleotides PS417_22060: PS417_22060 - hypothetical protein, at 4,779,828 to 4,780,205 _22060 PS417_22065: PS417_22065 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) (from data), at 4,780,320 to 4,782,011 _22065 PS417_22070: PS417_22070 - long-chain fatty acid--CoA ligase, at 4,782,407 to 4,784,095 _22070 PS417_22075: PS417_22075 - alpha/beta hydrolase, at 4,784,306 to 4,785,250 _22075 PS417_22080: PS417_22080 - 3-hydroxybutyryl-CoA dehydratase, at 4,785,308 to 4,785,778 _22080
Group Condition PS417_22060 PS417_22065 PS417_22070 PS417_22075 PS417_22080
carbon source octanoate (C) -0.5 -5.6 -0.3 +0.6 N.D.
carbon source octanoate (C) +0.1 -5.4 -0.3 +0.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -3.9 -0.0 +0.1 +0.0 N.D.
carbon source L-Valine (C) -3.9 -0.2 -0.1 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -4.1 +0.1 +0.7 -0.3 N.D.
phage P. simiae ORA MOI 0.1 -3.9 +0.5 +0.1 +0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.2 -0.4 +0.1 -0.4 N.D.
seeds Growth on radish seeds for 72 hours -2.4 +0.1 -0.1 -0.4 N.D.
phage P. simiae ORA MOI 10 -3.1 +0.6 -0.3 -0.1 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.5 -0.7 -0.2 -0.1 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.8 -0.3 +0.1 -0.5 N.D.
seeds Growth on radish seeds for 72 hours -2.3 +0.2 -0.0 -0.3 N.D.
phage P. simiae OR Antrim MOI 1 -2.8 +0.6 +0.2 -0.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -2.0 -0.3 +0.2 -0.0 N.D.
phage P. simiae OR Antrim MOI 10 -2.1 +0.5 -0.0 -0.3 N.D.
phage P. simiae Grant Run MOI 10 -1.9 +0.5 -0.3 -0.0 N.D.
phage P. simiae Grant Run MOI 10 -1.8 +0.9 -0.3 -0.3 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.9 -0.1 +0.3 +0.2 N.D.
phage P. simiae Grant Run MOI 0.1 -1.9 +0.5 +0.2 -0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.1 +0.3 +0.1 +0.2 N.D.
phage JP1 MOI 1 -1.8 +0.7 -0.1 -0.0 N.D.
solid stress Fraxetin 3 mM; solid stress +0.8 -1.7 -0.3 -0.0 N.D.
phage P. simiae OR1 MOI 0.1 -1.4 +0.7 +0.2 +0.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.0 +0.9 +0.3 -0.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.5 +0.2 -0.3 -0.4 N.D.
phage P. simiae ORA MOI 1 +1.5 +1.0 -0.5 -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.8 +0.2 +0.2 -0.3 N.D.
stress Doxycycline hyclate 0.001 mg/ml +1.2 +1.5 -0.1 -0.4 N.D.
phage JP1 MOI 10 +1.2 +1.1 -0.1 +0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.7 +0.2 +0.2 +0.9 N.D.
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