Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_21975 and PS417_21980 are separated by 158 nucleotidesPS417_21980 and PS417_21985 are separated by 84 nucleotidesPS417_21985 and PS417_21990 are separated by 213 nucleotidesPS417_21990 and PS417_21995 are separated by 312 nucleotides PS417_21975: PS417_21975 - glutamate-pyruvate aminotransferase, at 4,764,915 to 4,766,126 _21975 PS417_21980: PS417_21980 - permease DsdX, at 4,766,285 to 4,767,637 _21980 PS417_21985: PS417_21985 - gluconokinase, at 4,767,722 to 4,768,255 _21985 PS417_21990: PS417_21990 - LacI family transcriptional regulator, at 4,768,469 to 4,769,500 _21990 PS417_21995: PS417_21995 - hypothetical protein, at 4,769,813 to 4,770,046 _21995
Group Condition PS417_21975 PS417_21980 PS417_21985 PS417_21990 PS417_21995
agar plate interaction control Taped volatile agar plate with no fungus -0.8 -0.1 N.D. -1.8 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +0.1 N.D. -2.2 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 +0.5 N.D. -2.8 -0.3
nitrogen source Urea (N) -0.2 -1.8 N.D. -0.5 -0.0
carbon source D-Glucose (C) -0.0 -1.7 N.D. -0.6 -0.1
motility outer cut, LB soft agar motility assay -0.8 +0.0 N.D. -1.0 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 +0.2 N.D. -1.4 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.2 N.D. -1.4 -0.2
motility outer cut, LB soft agar motility assay -1.0 -0.3 N.D. -0.8 -0.0
carbon source D-Gluconic Acid (C) -0.3 -1.3 N.D. +0.2 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.4 N.D. -1.0 +0.5
stress methylglyoxal 0.032 vol% -0.5 +0.3 N.D. -1.2 -0.1
nitrogen source Ammonium chloride (N) -0.2 -1.9 N.D. +0.8 +0.0
carbon source D-Glucose-6-Phosphate (C) -0.2 -1.2 N.D. +0.6 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 -0.3 N.D. -0.9 +0.5
phage P. simiae OR Antrim MOI 0.1 -0.3 -0.6 N.D. +0.7 -0.8
soil soil sample 8; outgrowth in LB -0.3 +0.1 N.D. -1.3 +0.6
carbon source D-Glucose (C) -0.1 -1.3 N.D. +1.1 -0.3
seeds Growth on radish seeds for 24 hours -0.2 +0.4 N.D. -1.3 +0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.4 +0.8 N.D. +0.8 -0.8
phage P. simiae OR1 MOI 0.1 +0.1 -0.5 N.D. +1.3 -0.4
carbon source 2'-Deoxyinosine 5 mM (C) +0.2 -0.3 N.D. +1.1 -0.5
carbon source L-Valine (C) -0.3 -0.6 N.D. +1.2 +0.2
phage P. simiae OR Antrim MOI 10 -0.4 -0.1 N.D. +1.6 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.4 N.D. -0.9 +1.2
carbon source D-Glucosamine Hydrochloride (C) +0.1 -0.3 N.D. +1.6 -0.2
carbon source D-Glucosamine Hydrochloride (C) +0.0 -0.5 N.D. +1.9 -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 -0.2 N.D. +1.0 +1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.1 -0.2 N.D. +0.4 +1.7
nitrogen source Adenine (N) +0.1 +2.1 N.D. +1.1 +0.2
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