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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_21975 and PS417_21980 are separated by 158 nucleotides
PS417_21980 and PS417_21985 are separated by 84 nucleotides
PS417_21985 and PS417_21990 are separated by 213 nucleotides
PS417_21990 and PS417_21995 are separated by 312 nucleotides
PS417_21975: PS417_21975 - glutamate-pyruvate aminotransferase, at 4,764,915 to 4,766,126
_21975
PS417_21980: PS417_21980 - permease DsdX, at 4,766,285 to 4,767,637
_21980
PS417_21985: PS417_21985 - gluconokinase, at 4,767,722 to 4,768,255
_21985
PS417_21990: PS417_21990 - LacI family transcriptional regulator, at 4,768,469 to 4,769,500
_21990
PS417_21995: PS417_21995 - hypothetical protein, at 4,769,813 to 4,770,046
_21995
Group
Condition
PS417
_21975
PS417
_21980
PS417
_21985
PS417
_21990
PS417
_21995
agar plate interaction control
Taped volatile agar plate with no fungus
-0.8
-0.1
N.D.
-1.8
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
+0.1
N.D.
-2.2
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
+0.5
N.D.
-2.8
-0.3
nitrogen source
Urea (N)
-0.2
-1.8
N.D.
-0.5
-0.0
carbon source
D-Glucose (C)
-0.0
-1.7
N.D.
-0.6
-0.1
motility
outer cut, LB soft agar motility assay
-0.8
+0.0
N.D.
-1.0
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
+0.2
N.D.
-1.4
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-0.2
N.D.
-1.4
-0.2
motility
outer cut, LB soft agar motility assay
-1.0
-0.3
N.D.
-0.8
-0.0
carbon source
D-Gluconic Acid (C)
-0.3
-1.3
N.D.
+0.2
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-0.4
N.D.
-1.0
+0.5
stress
methylglyoxal 0.032 vol%
-0.5
+0.3
N.D.
-1.2
-0.1
nitrogen source
Ammonium chloride (N)
-0.2
-1.9
N.D.
+0.8
+0.0
carbon source
D-Glucose-6-Phosphate (C)
-0.2
-1.2
N.D.
+0.6
-0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
-0.3
N.D.
-0.9
+0.5
phage
P. simiae OR Antrim MOI 0.1
-0.3
-0.6
N.D.
+0.7
-0.8
soil
soil sample 8; outgrowth in LB
-0.3
+0.1
N.D.
-1.3
+0.6
carbon source
D-Glucose (C)
-0.1
-1.3
N.D.
+1.1
-0.3
seeds
Growth on radish seeds for 24 hours
-0.2
+0.4
N.D.
-1.3
+0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.4
+0.8
N.D.
+0.8
-0.8
phage
P. simiae OR1 MOI 0.1
+0.1
-0.5
N.D.
+1.3
-0.4
carbon source
2'-Deoxyinosine 5 mM (C)
+0.2
-0.3
N.D.
+1.1
-0.5
carbon source
L-Valine (C)
-0.3
-0.6
N.D.
+1.2
+0.2
phage
P. simiae OR Antrim MOI 10
-0.4
-0.1
N.D.
+1.6
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.4
N.D.
-0.9
+1.2
carbon source
D-Glucosamine Hydrochloride (C)
+0.1
-0.3
N.D.
+1.6
-0.2
carbon source
D-Glucosamine Hydrochloride (C)
+0.0
-0.5
N.D.
+1.9
-0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.1
-0.2
N.D.
+1.0
+1.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.1
-0.2
N.D.
+0.4
+1.7
nitrogen source
Adenine (N)
+0.1
+2.1
N.D.
+1.1
+0.2
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