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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_21965 and PS417_21970 are separated by 301 nucleotides
PS417_21970 and PS417_21975 are separated by 195 nucleotides
PS417_21975 and PS417_21980 are separated by 158 nucleotides
PS417_21980 and PS417_21985 are separated by 84 nucleotides
PS417_21965: PS417_21965 - GNAT family acetyltransferase, at 4,763,417 to 4,763,935
_21965
PS417_21970: PS417_21970 - AraC family transcriptional regulator, at 4,764,237 to 4,764,719
_21970
PS417_21975: PS417_21975 - glutamate-pyruvate aminotransferase, at 4,764,915 to 4,766,126
_21975
PS417_21980: PS417_21980 - permease DsdX, at 4,766,285 to 4,767,637
_21980
PS417_21985: PS417_21985 - gluconokinase, at 4,767,722 to 4,768,255
_21985
Group
Condition
PS417
_21965
PS417
_21970
PS417
_21975
PS417
_21980
PS417
_21985
nitrogen source
Ammonium chloride (N)
+0.1
N.D.
-0.2
-1.9
N.D.
nitrogen source
Urea (N)
+0.2
N.D.
-0.2
-1.8
N.D.
carbon source
D-Glucose (C)
-0.1
N.D.
-0.0
-1.7
N.D.
soil
soil sample 6; outgrowth in LB
-0.4
N.D.
-0.4
-0.8
N.D.
carbon source
D-Gluconic Acid (C)
-0.0
N.D.
-0.3
-1.3
N.D.
carbon source
D-Glucose (C)
+0.0
N.D.
-0.1
-1.5
N.D.
soil
soil sample 5; outgrowth in LB
-0.5
N.D.
-0.2
-0.8
N.D.
carbon source
D-Glucose (C)
-0.0
N.D.
-0.1
-1.3
N.D.
carbon source
D-Glucose-6-Phosphate (C)
+0.1
N.D.
-0.2
-1.2
N.D.
rhizosphere
rhizosphere sample 1; outgrowth in LB
-0.1
N.D.
-0.4
-0.7
N.D.
motility
outer cut, LB soft agar motility assay
-0.0
N.D.
-1.0
-0.3
N.D.
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.0
N.D.
-0.2
-1.0
N.D.
carbon source
L-Valine (C)
-0.4
N.D.
-0.3
-0.6
N.D.
soil
soil sample 3; outgrowth in LB
-0.2
N.D.
-0.2
-0.7
N.D.
carbon source
D-Gluconic Acid (C)
+0.1
N.D.
-0.1
-1.1
N.D.
motility
outer cut, LB soft agar motility assay
+0.0
N.D.
-0.9
-0.2
N.D.
nitrogen source
nitrate (N)
+0.1
N.D.
+0.0
-1.1
N.D.
motility
inner cut, LB soft agar motility assay
+0.1
N.D.
-0.9
-0.2
N.D.
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
N.D.
+0.1
-1.1
N.D.
carbon source
D-Glucose-6-Phosphate (C)
+0.2
N.D.
-0.3
-0.7
N.D.
phage
P. simiae OR Antrim MOI 0.1
+0.2
N.D.
-0.3
-0.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
N.D.
-0.7
+0.2
N.D.
phage
JP1 MOI 1
-0.4
N.D.
-0.4
+0.4
N.D.
stress
methylglyoxal 0.032 vol%
+0.4
N.D.
-0.5
+0.3
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.0
N.D.
-0.4
+0.8
N.D.
stress
Gentamicin 0.008 mg/ml
+0.8
N.D.
-0.4
+0.2
N.D.
phage
JP1 MOI 10
+0.1
N.D.
-0.2
+0.8
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.2
N.D.
+0.3
+0.7
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.8
N.D.
+0.2
+0.4
N.D.
nitrogen source
Adenine (N)
+0.0
N.D.
+0.1
+2.1
N.D.
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