Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_21845 and PS417_21850 overlap by 4 nucleotidesPS417_21850 and PS417_21855 are separated by 80 nucleotidesPS417_21855 and PS417_21860 overlap by 4 nucleotidesPS417_21860 and PS417_21865 overlap by 4 nucleotides PS417_21845: PS417_21845 - membrane protein, at 4,743,935 to 4,744,453 _21845 PS417_21850: PS417_21850 - LysR family transcriptional regulator, at 4,744,450 to 4,745,328 _21850 PS417_21855: PS417_21855 - hypothetical protein, at 4,745,409 to 4,746,110 _21855 PS417_21860: PS417_21860 - hypothetical protein, at 4,746,107 to 4,746,430 _21860 PS417_21865: PS417_21865 - hypothetical protein, at 4,746,427 to 4,746,750 _21865
Group Condition PS417_21845 PS417_21850 PS417_21855 PS417_21860 PS417_21865
soil soil sample 4; outgrowth in LB -0.4 -2.1 -0.4 N.D. -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.8 -1.0 -1.6 N.D. +0.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 -0.7 -1.0 N.D. -0.7
nitrogen source Adenine (N) -0.4 -1.6 -0.3 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 -1.2 -0.9 N.D. +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 -2.4 -0.2 N.D. +0.4
soil soil sample 5; outgrowth in LB +0.1 -2.7 +0.5 N.D. +0.1
phage JP1 MOI 0.1 -0.1 -0.8 -1.3 N.D. +0.4
stress Gentamicin 0.008 mg/ml -0.8 -1.4 +0.3 N.D. +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.8 -1.3 N.D. +0.7
rhizosphere rhizosphere sample 2; outgrowth in LB -0.7 +0.1 -1.3 N.D. +0.5
nophagecontrol Only library +0.0 -1.5 -0.4 N.D. +0.6
phage Wcs_1 -1.2 +1.2 -0.7 N.D. -0.1
seeds Growth on radish seeds for 72 hours -1.1 +1.4 -0.8 N.D. -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -1.4 +0.3 N.D. -0.3
seeds Growth on radish seeds for 24 hours -0.3 +0.9 -1.1 N.D. -0.3
agar plate interaction control Taped volatile agar plate with no fungus +0.4 -1.7 +0.2 N.D. +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.0 -0.9 +0.7 N.D. +0.6
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +1.1 -1.1 -0.3 N.D. -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 -1.1 +0.6 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +1.0 -1.2 -0.2 N.D. +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 +0.8 -1.0 N.D. -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 +0.5 -0.9 N.D. +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.9 -0.8 N.D. +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.2 +0.9 -0.1 N.D. -0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 +2.4 -0.5 N.D. +0.3
seeds Growth on radish seeds for 72 hours +0.7 +0.9 +0.7 N.D. +0.1
phage P. simiae ORA MOI 0.1 +0.6 +1.3 +0.3 N.D. +0.2
carbon source L-Valine (C) +1.5 +1.2 -0.8 N.D. +0.6
seeds Growth on radish seeds for 72 hours +0.7 +3.2 +0.2 N.D. +0.1
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