Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_21765 and PS417_21770 are separated by 296 nucleotidesPS417_21770 and PS417_21775 are separated by 251 nucleotidesPS417_21775 and PS417_21780 are separated by 193 nucleotidesPS417_21780 and PS417_21785 are separated by 106 nucleotides PS417_21765: PS417_21765 - ribonucleotide-diphosphate reductase subunit beta, at 4,726,177 to 4,727,427 _21765 PS417_21770: PS417_21770 - HAD family hydrolase, at 4,727,724 to 4,728,377 _21770 PS417_21775: PS417_21775 - LysR family transcriptional regulator, at 4,728,629 to 4,729,588 _21775 PS417_21780: PS417_21780 - argininosuccinate lyase, at 4,729,782 to 4,731,209 _21780 PS417_21785: PS417_21785 - ABC transporter permease, at 4,731,316 to 4,732,194 _21785
Group Condition PS417_21765 PS417_21770 PS417_21775 PS417_21780 PS417_21785
soil soil sample 2; outgrowth in LB N.D. N.D. -0.0 -1.9 -0.2
liquid stress Fraxetin 3 mM; liquid stress N.D. N.D. +0.0 -1.3 +0.0
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. +0.1 -1.2 -0.2
phage JP1 MOI 10 N.D. N.D. +0.2 -0.7 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.1 -1.1 +0.0
phage Wcs_0.1 N.D. N.D. +0.4 -0.7 -0.4
phage JP1 MOI 10 N.D. N.D. +0.0 -1.0 +0.3
phage P. simiae ORA MOI 0.1 N.D. N.D. -0.5 -0.4 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.4 -0.9 -0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.4 -0.4 -0.5
solid stress Fraxetin 3 mM; solid stress N.D. N.D. +0.4 -1.0 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.2 -0.8 +0.2
motility inner cut, LB soft agar motility assay N.D. N.D. +0.2 -0.9 +0.4
soil soil sample 4; outgrowth in LB N.D. N.D. +0.3 -0.6 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.3 -0.7 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.4 +0.6 -0.3
phage P. simiae Grant Run MOI 0.1 N.D. N.D. -0.4 +0.7 -0.3
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. -0.0 +0.8 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.0 -0.5 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.4 -0.7 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.3 -0.3 +0.5
soil soil sample 5; outgrowth in LB N.D. N.D. +0.2 +0.8 -0.3
solid stress 80% Methanol 2 mM; solid stress N.D. N.D. -0.1 +0.9 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.2 +1.0 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.0 +0.7 +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. +0.3 +0.7 +0.2
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. N.D. +0.1 +1.2 -0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. -0.1 +1.4 +0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. +0.3 +0.6 +0.5
seeds Growth on radish seeds for 72 hours N.D. N.D. +0.2 +1.6 +0.6
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