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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_02145 and PS417_02150 are separated by 50 nucleotides
PS417_02150 and PS417_02155 are separated by 243 nucleotides
PS417_02155 and PS417_02160 are separated by 278 nucleotides
PS417_02160 and PS417_02165 overlap by 4 nucleotides
PS417_02145: PS417_02145 - potassium transporter KefA, at 477,221 to 480,559
_02145
PS417_02150: PS417_02150 - potassium/proton antiporter, at 480,610 to 482,352
_02150
PS417_02155: PS417_02155 - chemotaxis protein, at 482,596 to 484,221
_02155
PS417_02160: PS417_02160 - hypothetical protein, at 484,500 to 484,730
_02160
PS417_02165: PS417_02165 - ZapA, at 484,727 to 485,020
_02165
Group
Condition
PS417
_02145
PS417
_02150
PS417
_02155
PS417
_02160
PS417
_02165
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-2.8
-1.4
-0.5
-1.0
+0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-2.2
-1.6
+0.0
-0.8
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-2.1
-1.3
+0.3
-0.5
-0.0
nophagecontrol
Only library
-0.4
-0.2
-0.0
-2.7
-0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-1.7
-1.1
-0.0
-0.6
+0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-2.2
-1.2
-0.0
+0.3
+0.3
phage
P. simiae Grant Run MOI 10
+0.0
-0.3
+0.4
-1.4
-1.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-2.0
-1.6
-0.2
+0.8
+0.5
seeds
Growth on radish seeds for 72 hours
+0.3
+0.3
-0.7
-3.0
+0.6
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-1.8
-1.0
+0.4
+0.0
+0.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-1.3
-0.8
+0.3
-0.0
-0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-2.0
-0.8
+0.0
+0.8
-0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-2.1
-1.4
-0.3
+1.0
+0.8
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-1.7
-0.8
+0.3
+0.4
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
-0.4
+0.1
-1.9
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
-0.2
+0.3
-1.2
-0.5
carbon source
L-Valine (C)
+0.2
+1.3
-0.1
-1.7
-0.3
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
-0.2
-0.1
+0.6
-1.3
+0.7
root
root sample 6; outgrowth in LB
+0.1
+0.4
+0.9
-2.1
+0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.0
+0.2
+0.5
-1.4
+0.8
seeds
Growth on radish seeds for 72 hours
-0.1
+0.2
+0.0
-1.2
+1.9
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.1
+0.8
+0.2
+1.0
-0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-0.4
+0.4
+0.6
+1.2
carbon source
L-Valine (C)
+0.2
+1.2
-0.9
+0.7
+0.6
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x
-0.3
-0.2
+0.8
+1.3
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.1
+0.2
-0.3
+2.4
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.7
+0.1
+2.3
+0.0
+0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.3
+0.5
-0.1
+1.7
+0.0
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x
+0.1
-0.0
+0.4
+1.1
+0.9
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.7
+0.3
+3.5
-0.2
+0.6
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