Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_02145 and PS417_02150 are separated by 50 nucleotidesPS417_02150 and PS417_02155 are separated by 243 nucleotidesPS417_02155 and PS417_02160 are separated by 278 nucleotidesPS417_02160 and PS417_02165 overlap by 4 nucleotides PS417_02145: PS417_02145 - potassium transporter KefA, at 477,221 to 480,559 _02145 PS417_02150: PS417_02150 - potassium/proton antiporter, at 480,610 to 482,352 _02150 PS417_02155: PS417_02155 - chemotaxis protein, at 482,596 to 484,221 _02155 PS417_02160: PS417_02160 - hypothetical protein, at 484,500 to 484,730 _02160 PS417_02165: PS417_02165 - ZapA, at 484,727 to 485,020 _02165
Group Condition PS417_02145 PS417_02150 PS417_02155 PS417_02160 PS417_02165
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.8 -1.4 -0.5 -1.0 +0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.2 -1.6 +0.0 -0.8 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.1 -1.3 +0.3 -0.5 -0.0
nophagecontrol Only library -0.4 -0.2 -0.0 -2.7 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.7 -1.1 -0.0 -0.6 +0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -2.2 -1.2 -0.0 +0.3 +0.3
phage P. simiae Grant Run MOI 10 +0.0 -0.3 +0.4 -1.4 -1.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.0 -1.6 -0.2 +0.8 +0.5
seeds Growth on radish seeds for 72 hours +0.3 +0.3 -0.7 -3.0 +0.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.8 -1.0 +0.4 +0.0 +0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.3 -0.8 +0.3 -0.0 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -2.0 -0.8 +0.0 +0.8 -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.1 -1.4 -0.3 +1.0 +0.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.7 -0.8 +0.3 +0.4 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 -0.4 +0.1 -1.9 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 -0.2 +0.3 -1.2 -0.5
carbon source L-Valine (C) +0.2 +1.3 -0.1 -1.7 -0.3
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -0.2 -0.1 +0.6 -1.3 +0.7
root root sample 6; outgrowth in LB +0.1 +0.4 +0.9 -2.1 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 +0.2 +0.5 -1.4 +0.8
seeds Growth on radish seeds for 72 hours -0.1 +0.2 +0.0 -1.2 +1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.8 +0.2 +1.0 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.4 +0.4 +0.6 +1.2
carbon source L-Valine (C) +0.2 +1.2 -0.9 +0.7 +0.6
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x -0.3 -0.2 +0.8 +1.3 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.1 +0.2 -0.3 +2.4 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.7 +0.1 +2.3 +0.0 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 +0.5 -0.1 +1.7 +0.0
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x +0.1 -0.0 +0.4 +1.1 +0.9
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 +0.3 +3.5 -0.2 +0.6
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