Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_21645 and PS417_21650 are separated by 62 nucleotidesPS417_21650 and PS417_21655 are separated by 174 nucleotidesPS417_21655 and PS417_21660 are separated by 95 nucleotidesPS417_21660 and PS417_21665 are separated by 146 nucleotides PS417_21645: PS417_21645 - tRNA-Ser, at 4,694,535 to 4,694,625 _21645 PS417_21650: PS417_21650 - carbon storage regulator, at 4,694,688 to 4,694,876 _21650 PS417_21655: PS417_21655 - aspartate kinase, at 4,695,051 to 4,696,292 _21655 PS417_21660: PS417_21660 - alanyl-tRNA synthetase, at 4,696,388 to 4,699,006 _21660 PS417_21665: PS417_21665 - threonine aldolase, at 4,699,153 to 4,700,157 _21665
Group Condition PS417_21645 PS417_21650 PS417_21655 PS417_21660 PS417_21665
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -3.1 N.D. -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -3.1 N.D. -0.3
supernatant control Vogels_fungal_media 0.1X N.D. N.D. -3.3 N.D. -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -3.0 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -3.1 N.D. -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -3.1 N.D. -0.0
stress methylglyoxal 0.032 vol% N.D. N.D. -3.0 N.D. -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -3.1 N.D. +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.7 N.D. -0.3
phage JP1 MOI 1 N.D. N.D. -2.7 N.D. -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. -2.9 N.D. -0.1
carbon source L-Arginine (C) N.D. N.D. -3.0 N.D. +0.0
carbon source L-Arginine (C) N.D. N.D. -2.7 N.D. -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.3 N.D. -0.4
soil soil sample 4; outgrowth in LB N.D. N.D. -2.4 N.D. -0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. -2.3 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.6 N.D. +0.0
soil soil sample 7; outgrowth in LB N.D. N.D. -2.7 N.D. +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.1 N.D. -0.4
phage P. simiae OR Antrim MOI 1 N.D. N.D. -2.5 N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.2 N.D. -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. -2.2 N.D. -0.1
carbon source L-Citrulline (C) N.D. N.D. -2.5 N.D. +0.2
stress Choline chloride 300 mM N.D. N.D. -2.2 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.1 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.3 N.D. +0.1
liquid stress 80% Methanol 3 mM; liquid stress N.D. N.D. -2.4 N.D. +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. -2.2 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +2.4 N.D. -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +3.0 N.D. +0.0
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