Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_21620 and PS417_21625 are separated by 386 nucleotidesPS417_21625 and PS417_21630 are separated by 102 nucleotidesPS417_21630 and PS417_21635 are separated by 340 nucleotidesPS417_21635 and PS417_21640 are separated by 72 nucleotides PS417_21620: PS417_21620 - hypothetical protein, at 4,691,423 to 4,691,602 _21620 PS417_21625: PS417_21625 - DNA-binding protein, at 4,691,989 to 4,692,315 _21625 PS417_21630: PS417_21630 - magnesium transporter, at 4,692,418 to 4,693,860 _21630 PS417_21635: PS417_21635 - tRNA-Arg, at 4,694,201 to 4,694,277 _21635 PS417_21640: PS417_21640 - tRNA-Arg, at 4,694,350 to 4,694,426 _21640
Group Condition PS417_21620 PS417_21625 PS417_21630 PS417_21635 PS417_21640
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -5.3 -2.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -5.3 -2.4 N.D. N.D.
solid stress Fraxetin 3 mM; solid stress N.D. -4.3 -1.4 N.D. N.D.
agar plate interaction control Taped volatile agar plate with no fungus N.D. -2.0 -3.5 N.D. N.D.
motility outer cut, LB soft agar motility assay N.D. -3.3 -2.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.2 -4.0 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.1 -3.9 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.8 -4.1 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -3.5 -1.3 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.0 -3.6 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -2.1 -2.2 N.D. N.D.
phage P. simiae OR Antrim MOI 0.1 N.D. -4.0 -0.3 N.D. N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.8 -2.2 N.D. N.D.
motility inner cut, LB soft agar motility assay N.D. -0.9 -3.0 N.D. N.D.
phage P. simiae ORA MOI 0.1 N.D. -4.3 +0.3 N.D. N.D.
phage P. simiae Grant Run MOI 0.1 N.D. -4.1 +0.3 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 -4.0 N.D. N.D.
phage P. simiae ORA MOI 10 N.D. -4.3 +0.8 N.D. N.D.
soil soil sample 2; outgrowth in LB N.D. +0.6 -3.5 N.D. N.D.
phage JP1 MOI 10 N.D. -3.4 +0.6 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +1.1 -3.6 N.D. N.D.
soil soil sample 8; outgrowth in LB N.D. +1.1 -3.6 N.D. N.D.
soil soil sample 6; outgrowth in LB N.D. +1.3 -3.5 N.D. N.D.
soil soil sample 7; outgrowth in LB N.D. +0.9 -3.1 N.D. N.D.
soil soil sample 4; outgrowth in LB N.D. +2.2 -2.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +2.3 -2.3 N.D. N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. +3.0 -1.7 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +2.9 -1.4 N.D. N.D.
root root sample 6; outgrowth in LB N.D. +3.2 -1.4 N.D. N.D.
stress Cobalt chloride 0.16 mM N.D. -0.3 +10.0 N.D. N.D.
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PS417_21620
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PS417_21625
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PS417_21635
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PS417_21640
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