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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_21455 and PS417_21460 are separated by 13 nucleotides
PS417_21460 and PS417_21465 are separated by 105 nucleotides
PS417_21465 and PS417_21470 are separated by 133 nucleotides
PS417_21470 and PS417_21475 are separated by 21 nucleotides
PS417_21455: PS417_21455 - 50S ribosomal protein L32, at 4,658,206 to 4,658,388
_21455
PS417_21460: PS417_21460 - metal-binding protein, at 4,658,402 to 4,658,932
_21460
PS417_21465: PS417_21465 - septum formation inhibitor Maf, at 4,659,038 to 4,659,616
_21465
PS417_21470: PS417_21470 - peptidase S49, at 4,659,750 to 4,660,733
_21470
PS417_21475: PS417_21475 - HAD family hydrolase, at 4,660,755 to 4,661,417
_21475
Group
Condition
PS417
_21455
PS417
_21460
PS417
_21465
PS417
_21470
PS417
_21475
nitrogen source
Glycine (N)
N.D.
N.D.
-0.3
+0.0
-1.6
phage
JP1 MOI 1
N.D.
N.D.
+0.2
-0.2
-1.9
seeds
Growth on radish seeds for 72 hours
N.D.
N.D.
-0.5
-0.4
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.1
+0.3
-2.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.6
-0.1
-0.8
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
N.D.
-0.3
-0.3
-0.9
seeds
Growth on radish seeds for 24 hours
N.D.
N.D.
-0.7
-0.1
-0.7
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
N.D.
-0.8
+0.1
-0.6
phage
P. simiae OR Antrim MOI 1
N.D.
N.D.
-0.5
-0.0
-0.9
stress
Gentamicin 0.008 mg/ml
N.D.
N.D.
+0.5
-0.5
-1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.1
+0.1
-1.1
soil
soil sample 6; outgrowth in LB
N.D.
N.D.
-0.3
+0.3
-1.2
liquid stress
Scopoletin 2 mM; liquid stress
N.D.
N.D.
+0.3
+0.0
-1.3
soil
soil sample 5; outgrowth in LB
N.D.
N.D.
+0.5
+0.2
-1.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.4
+0.2
-1.2
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
N.D.
-1.3
+0.2
+0.7
rhizosphere
rhizosphere sample 8; outgrowth in LB
N.D.
N.D.
-0.0
+0.5
-0.9
liquid stress
Scopoletin 3 mM; liquid stress
N.D.
N.D.
+0.5
+0.2
-0.8
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
N.D.
-0.9
+0.3
+0.6
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
N.D.
-0.5
+0.0
+0.8
phage
JP1 MOI 10
N.D.
N.D.
-0.4
-0.2
+1.0
ecofab
LB in EcoFAB_3.5mL
N.D.
N.D.
-0.3
+0.2
+0.8
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
N.D.
N.D.
-0.3
+0.1
+1.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
N.D.
N.D.
-0.5
-0.2
+1.8
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
N.D.
-0.0
-0.1
+1.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
N.D.
+1.2
+0.1
-0.1
motility
inner cut, LB soft agar motility assay
N.D.
N.D.
-0.2
+0.3
+1.4
rhizosphere
rhizosphere sample 2; outgrowth in LB
N.D.
N.D.
-0.0
+0.7
+0.8
phage
P. simiae OR1 MOI 0.1
N.D.
N.D.
-0.1
+0.2
+1.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.3
+0.1
+1.3
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