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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_21290 and PS417_21295 are separated by 70 nucleotides
PS417_21295 and PS417_21300 are separated by 2 nucleotides
PS417_21300 and PS417_21305 are separated by 507 nucleotides
PS417_21305 and PS417_21310 are separated by 177 nucleotides
PS417_21290: PS417_21290 - ABC transporter substrate-binding protein, at 4,626,905 to 4,627,720
_21290
PS417_21295: PS417_21295 - electron transfer flavoprotein subunit beta, at 4,627,791 to 4,628,720
_21295
PS417_21300: PS417_21300 - electron transporter RnfB, at 4,628,723 to 4,629,472
_21300
PS417_21305: PS417_21305 - electron transfer flavoprotein-ubiquinone oxidoreductase, at 4,629,980 to 4,631,644
_21305
PS417_21310: PS417_21310 - AraC family transcriptional regulator, at 4,631,822 to 4,632,715
_21310
Group
Condition
PS417
_21290
PS417
_21295
PS417
_21300
PS417
_21305
PS417
_21310
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.8
N.D.
N.D.
N.D.
-0.4
carbon source
L-Isoleucine (C)
-0.5
N.D.
N.D.
N.D.
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
N.D.
N.D.
N.D.
-0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
N.D.
N.D.
N.D.
-0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.0
N.D.
N.D.
N.D.
-0.7
seeds
Growth on radish seeds for 24 hours
-0.6
N.D.
N.D.
N.D.
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
N.D.
N.D.
N.D.
+0.0
seeds
Growth on radish seeds for 72 hours
+0.1
N.D.
N.D.
N.D.
-0.4
phage
P. simiae ORA MOI 10
-0.4
N.D.
N.D.
N.D.
+0.1
phage
P. simiae OR Antrim MOI 1
+0.3
N.D.
N.D.
N.D.
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
N.D.
N.D.
N.D.
+0.3
carbon source
2-Deoxy-D-Ribose (C)
-0.4
N.D.
N.D.
N.D.
+0.3
stress
6-methoxy-2(3H)-benzoxazolone 2.5 mM
+0.3
N.D.
N.D.
N.D.
-0.3
solid stress
80% Methanol 2 mM; solid stress
+0.3
N.D.
N.D.
N.D.
-0.3
phage
Wcs_0.1
+0.3
N.D.
N.D.
N.D.
-0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.4
N.D.
N.D.
N.D.
-0.3
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
+0.4
N.D.
N.D.
N.D.
-0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
N.D.
N.D.
N.D.
-0.3
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
+0.4
N.D.
N.D.
N.D.
-0.3
soil
soil sample 7; outgrowth in LB
+0.4
N.D.
N.D.
N.D.
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
N.D.
N.D.
N.D.
+0.5
stress
Nalidixic 0.025 mg/ml
-0.1
N.D.
N.D.
N.D.
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
N.D.
N.D.
-0.1
supernatant control
Vogels_fungal_media 0.1X
+0.5
N.D.
N.D.
N.D.
+0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.2
N.D.
N.D.
N.D.
+0.3
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.4
N.D.
N.D.
N.D.
+0.1
stress
1-ethyl-3-methylimidazolium acetate 35 mM
+0.4
N.D.
N.D.
N.D.
+0.2
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.4
N.D.
N.D.
N.D.
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.8
N.D.
N.D.
N.D.
-0.0
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.6
N.D.
N.D.
N.D.
+0.4
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