Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_21255 and PS417_21260 are separated by 103 nucleotidesPS417_21260 and PS417_21265 are separated by 41 nucleotidesPS417_21265 and PS417_21270 are separated by 162 nucleotidesPS417_21270 and PS417_21275 are separated by 104 nucleotides PS417_21255: PS417_21255 - hypothetical protein, at 4,622,017 to 4,622,253 _21255 PS417_21260: PS417_21260 - hypothetical protein, at 4,622,357 to 4,622,962 _21260 PS417_21265: PS417_21265 - hypothetical protein, at 4,623,004 to 4,623,258 _21265 PS417_21270: PS417_21270 - translation initiation factor 2, at 4,623,421 to 4,623,906 _21270 PS417_21275: PS417_21275 - GntR family transcriptional regulator, at 4,624,011 to 4,625,450 _21275
Group Condition PS417_21255 PS417_21260 PS417_21265 PS417_21270 PS417_21275
carbon source L-Valine (C) +0.8 -0.3 N.D. -0.5 -4.8
carbon source L-Valine (C) -0.1 +0.2 N.D. -0.2 -4.4
carbon source L-Isoleucine (C) -0.8 -0.0 N.D. -0.2 -2.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.2 N.D. -4.0 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.1 N.D. -2.5 +0.1
phage JP1 MOI 10 -2.8 +0.2 N.D. +0.5 -0.3
nophagecontrol Only library -1.7 +0.0 N.D. -0.3 -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 -0.1 N.D. -1.5 -0.7
phage JP1 MOI 1 -1.4 -0.1 N.D. -0.3 -0.5
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 -0.3 N.D. -1.5 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.2 N.D. -1.1 -1.2
solid stress Fraxetin 3 mM; solid stress +0.2 -0.1 N.D. -0.5 -1.5
soil soil sample 5; outgrowth in LB -1.1 +0.2 N.D. -1.2 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 +0.0 N.D. -1.5 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 -0.0 N.D. -1.8 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.6 +0.3 N.D. -0.7 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.1 -0.1 N.D. -0.9 +0.4
seeds Growth on radish seeds for 72 hours -0.2 +0.5 N.D. +1.4 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -1.2 +0.5 N.D. +2.4 -0.3
stress R2A with Fusaric 120 ug/mL +0.3 -0.2 N.D. +2.1 -0.7
stress R2A with Fusaric 120 ug/mL +0.1 +0.1 N.D. +1.9 -0.6
stress R2A with Fusaric 120 ug/mL -0.1 +0.1 N.D. +2.3 -0.7
stress R2A with Fusaric 120 ug/mL +0.1 -0.0 N.D. +2.4 -0.7
stress R2A with Fusaric 120 ug/mL +0.3 +0.1 N.D. +2.2 -0.6
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.3 +0.0 N.D. -0.3 +2.5
stress R2A with Fusaric 120 ug/mL +0.5 +0.2 N.D. +2.1 -0.5
rhizosphere rhizosphere sample 8; outgrowth in LB +0.6 +0.1 N.D. +0.6 +1.3
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.2 +0.0 N.D. -0.3 +3.0
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -0.2 +0.3 N.D. +0.1 +3.0
soil soil sample 4; outgrowth in LB +0.3 -0.0 N.D. +0.0 +3.1
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