Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_21215 and PS417_21220 are separated by 16 nucleotidesPS417_21220 and PS417_21230 are separated by 646 nucleotidesPS417_21230 and PS417_21235 are separated by 132 nucleotidesPS417_21235 and PS417_21240 overlap by 4 nucleotides PS417_21215: PS417_21215 - 3-hydroxyacyl-CoA dehydrogenase, at 4,612,932 to 4,615,079 _21215 PS417_21220: PS417_21220 - acetyl-CoA acetyltransferase, at 4,615,096 to 4,616,301 _21220 PS417_21230: PS417_21230 - cytochrome C, at 4,616,948 to 4,617,751 _21230 PS417_21235: PS417_21235 - transglutaminase, at 4,617,884 to 4,618,687 _21235 PS417_21240: PS417_21240 - hypothetical protein, at 4,618,684 to 4,619,634 _21240
Group Condition PS417_21215 PS417_21220 PS417_21230 PS417_21235 PS417_21240
phage P. simiae ORA MOI 0.1 -0.3 -1.6 -0.2 -0.7 -0.4
stress methylglyoxal 0.032 vol% -0.1 -0.5 -0.4 -0.6 -0.5
carbon source 2'-Deoxyinosine 5 mM (C) -0.0 -0.4 -0.6 -1.0 -0.0
phage P. simiae OR Antrim MOI 1 -0.2 -0.3 -0.1 -0.9 -0.4
nophagecontrol Only library -0.3 -0.9 -0.4 -0.2 -0.2
soil soil sample 5; outgrowth in LB -0.9 -0.2 +0.1 -0.4 -0.4
phage P. simiae ORA MOI 10 -0.2 -0.9 -0.4 +0.1 -0.2
phage P. simiae OR Antrim MOI 0.1 -0.1 -0.6 -0.5 +0.4 -0.7
motility outer cut, LB soft agar motility assay +0.1 -0.3 +0.1 -0.8 -0.4
phage JP1 MOI 1 -0.0 -1.0 +0.3 -0.2 -0.2
phage P. simiae Grant Run MOI 10 -0.5 -0.3 -0.0 -0.6 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.2 -0.4 -0.6 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.2 -0.2 -1.0 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -1.2 -0.0 +0.7 +0.1
phage P. simiae Grant Run MOI 0.1 +0.0 +0.5 -0.4 -0.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.5 +0.5 -0.5 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.7 +1.0 -0.2 -0.4
soil soil sample 2; outgrowth in LB +0.5 +0.2 -0.2 -0.7 -0.1
agar plate interaction control Taped volatile agar plate with no fungus -0.3 -0.5 -0.1 +0.7 +0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.0 +0.8 -0.5 +0.2 -0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.9 -0.8 -0.7 +0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.1 +0.2 -1.1 +0.5 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.5 -0.3 -0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +1.0 -0.1 -0.3 +0.2
seeds Growth on radish seeds for 72 hours +0.1 +1.0 +0.2 -0.2 -0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.8 +0.1 -0.2 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 +0.7 -0.2 -0.2 +0.4
rhizosphere rhizosphere sample 2; outgrowth in LB -0.2 +1.3 +0.3 +0.3 -0.2
carbon source 2-Deoxy-D-Ribose (C) -0.1 +0.3 +0.3 +0.8 +0.2
root root sample 6; outgrowth in LB +0.0 +0.8 +0.6 +0.3 +0.3
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