Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_21205 and PS417_21210 overlap by 4 nucleotidesPS417_21210 and PS417_21215 are separated by 83 nucleotidesPS417_21215 and PS417_21220 are separated by 16 nucleotidesPS417_21220 and PS417_21230 are separated by 646 nucleotides PS417_21205: PS417_21205 - magnesium transporter, at 4,611,155 to 4,612,126 _21205 PS417_21210: PS417_21210 - amidotransferase, at 4,612,123 to 4,612,848 _21210 PS417_21215: PS417_21215 - 3-hydroxyacyl-CoA dehydrogenase, at 4,612,932 to 4,615,079 _21215 PS417_21220: PS417_21220 - acetyl-CoA acetyltransferase, at 4,615,096 to 4,616,301 _21220 PS417_21230: PS417_21230 - cytochrome C, at 4,616,948 to 4,617,751 _21230
Group Condition PS417_21205 PS417_21210 PS417_21215 PS417_21220 PS417_21230
soil soil sample 3; outgrowth in LB -0.1 -2.1 -0.4 -0.1 -0.5
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.1 -2.4 +0.2 -0.3 -0.2
phage P. simiae OR Antrim MOI 1 +0.4 -1.9 -0.2 -0.6 -0.3
carbon source Putrescine (C) +0.1 -2.3 +0.1 -0.1 -0.1
carbon source L-Arginine (C) -0.0 -2.1 +0.1 +0.0 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -2.0 -0.1 -0.2 +0.1
phage P. simiae ORA MOI 0.1 +0.1 -0.1 -0.3 -1.6 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -2.5 -0.1 +0.2 +0.2
soil soil sample 5; outgrowth in LB +0.2 -1.1 -0.9 -0.2 +0.1
carbon source Putrescine (C) +0.0 -1.9 +0.1 +0.1 -0.2
soil soil sample 2; outgrowth in LB +0.1 -2.4 +0.5 +0.2 -0.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.0 -2.1 +0.2 -0.1 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -1.7 +0.0 -0.3 +0.1
carbon source Gly-Glu (C) +0.1 -2.1 +0.1 +0.3 -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 -1.0 +0.1 +0.5 -0.6
soil soil sample 8; outgrowth in LB +0.0 -2.0 +0.5 +0.5 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 -1.6 +0.2 +0.4 +0.2
nophagecontrol Only library +0.2 +0.7 -0.3 -0.9 -0.4
rhizosphere rhizosphere sample 1; outgrowth in LB -0.0 -1.1 -0.4 +0.7 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -1.8 +0.5 +0.7 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.9 +0.2 -0.1 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -1.3 -0.1 +0.7 +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.1 +0.4 +0.9 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.9 +0.3 +0.7 +0.2
seeds Growth on radish seeds for 72 hours +0.7 -0.9 +0.1 +0.7 +0.1
rhizosphere rhizosphere sample 2; outgrowth in LB +0.4 -0.7 -0.2 +1.3 +0.3
root root sample 6; outgrowth in LB +0.6 -0.6 +0.0 +0.8 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 +1.1 -0.3 +1.1 +0.0
stress Gentamicin 0.008 mg/ml -0.1 +2.4 +0.0 -0.2 +0.2
seeds Growth on radish seeds for 72 hours +0.1 +1.3 +0.6 +0.4 -0.1
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