Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_02090 and PS417_02095 overlap by 8 nucleotidesPS417_02095 and PS417_02100 overlap by 4 nucleotidesPS417_02100 and PS417_02105 overlap by 4 nucleotidesPS417_02105 and PS417_02110 are separated by 9 nucleotides PS417_02090: PS417_02090 - glycosyl transferase, at 468,332 to 469,066 _02090 PS417_02095: PS417_02095 - AMP-binding protein, at 469,059 to 470,729 _02095 PS417_02100: PS417_02100 - membrane protein, at 470,726 to 471,271 _02100 PS417_02105: PS417_02105 - acyl carrier protein, at 471,268 to 471,522 _02105 PS417_02110: PS417_02110 - acyl carrier protein, at 471,532 to 471,801 _02110
Group Condition PS417_02090 PS417_02095 PS417_02100 PS417_02105 PS417_02110
phage P. simiae OR1 MOI 0.1 N.D. -0.3 -0.8 +0.1 -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.0 -0.3 -0.9 -1.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -0.0 -0.1 -0.5 -1.4
phage JP1 MOI 10 N.D. -0.3 -0.5 +0.7 -1.4
stress Gentamicin 0.008 mg/ml N.D. +0.3 -0.4 -0.9 -0.4
seeds Growth on radish seeds for 72 hours N.D. +0.0 +0.4 -0.3 -1.3
soil soil sample 4; outgrowth in LB N.D. +0.4 +0.3 +0.7 -2.1
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.5 -0.8 +0.7 +0.3
motility outer cut, LB soft agar motility assay N.D. +0.6 -0.2 +0.4 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 -0.1 -1.0 +0.8
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.4 +0.7 +0.3 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 +0.1 -1.0 +0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.1 +1.2 -0.5 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 +0.5 +0.7 -0.8
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.5 +0.7 +0.4 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.4 +0.5 -0.5 +0.6
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 +0.4 +1.0 -0.4
phage JP1 MOI 0.1 N.D. +0.1 -1.1 +0.9 +1.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.5 +0.8 -0.4 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.2 +0.6 +1.1 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 +0.7 +0.4 +0.7
seeds Growth on radish seeds for 72 hours N.D. +0.0 +1.1 -0.3 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 +0.3 +0.8 +0.7
soil soil sample 3; outgrowth in LB N.D. +0.5 +0.3 +0.5 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.4 +0.9 +0.5 +0.4
seeds Growth on radish seeds for 24 hours N.D. +0.7 +0.4 +0.5 +0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.9 -0.0 +1.5 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.6 +0.1 +0.9 +0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.7 +0.2 +2.1 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.8 -0.0 -0.2 +9.3
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