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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_20860 and PS417_20865 are separated by 176 nucleotides
PS417_20865 and PS417_20870 are separated by 87 nucleotides
PS417_20870 and PS417_20875 are separated by 259 nucleotides
PS417_20875 and PS417_20880 are separated by 2 nucleotides
PS417_20860: PS417_20860 - DNA polymerase III subunit gamma/tau, at 4,529,876 to 4,531,039
_20860
PS417_20865: PS417_20865 - NAD-dependent DNA ligase LigA, at 4,531,216 to 4,533,573
_20865
PS417_20870: PS417_20870 - cell division protein ZipA, at 4,533,661 to 4,534,524
_20870
PS417_20875: PS417_20875 - chromosome segregation protein SMC, at 4,534,784 to 4,538,272
_20875
PS417_20880: PS417_20880 - GntR family transcriptional regulator, at 4,538,275 to 4,538,934
_20880
Group
Condition
PS417
_20860
PS417
_20865
PS417
_20870
PS417
_20875
PS417
_20880
stress
Cisplatin 0.01 mg/ml
-0.4
N.D.
N.D.
-1.5
-1.9
phage
JP1 MOI 1
-0.0
N.D.
N.D.
+0.0
-3.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
N.D.
N.D.
-2.1
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
N.D.
-0.3
-2.8
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
N.D.
N.D.
-1.0
-1.8
motility
inner cut, LB soft agar motility assay
-0.2
N.D.
N.D.
-0.7
-1.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.3
N.D.
N.D.
-0.6
-1.6
liquid stress
Scopoletin 3 mM; liquid stress
+0.1
N.D.
N.D.
-0.7
-1.8
stress
nitrate 200 mM
-0.3
N.D.
N.D.
-0.6
-1.5
stress
Gentamicin 0.008 mg/ml
-0.8
N.D.
N.D.
-0.8
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
N.D.
N.D.
-0.9
-0.9
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
N.D.
N.D.
+0.2
-2.3
soil
soil sample 8; outgrowth in LB
-0.7
N.D.
N.D.
-0.4
-1.0
motility
outer cut, LB soft agar motility assay
+0.3
N.D.
N.D.
-1.0
-1.4
liquid stress
80% Methanol 2 mM; liquid stress
+0.0
N.D.
N.D.
-1.1
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
N.D.
N.D.
-0.5
-1.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.3
N.D.
N.D.
-0.4
-1.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
N.D.
N.D.
-1.8
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
N.D.
N.D.
-1.8
+0.4
phage
JP1 MOI 10
+0.3
N.D.
N.D.
+0.6
-2.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.2
N.D.
N.D.
+1.0
-1.5
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.3
N.D.
N.D.
+0.7
-1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
N.D.
N.D.
-1.6
+1.2
phage
P. simiae OR1 MOI 0.1
-0.6
N.D.
N.D.
-0.6
+1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.2
N.D.
N.D.
-0.9
+0.8
seeds
Growth on radish seeds for 72 hours
+0.5
N.D.
N.D.
-1.0
+1.1
carbon source
octanoate (C)
+0.1
N.D.
N.D.
+0.1
+1.9
carbon source
octanoate (C)
-0.1
N.D.
N.D.
+0.4
+1.9
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
N.D.
N.D.
+1.0
+1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.7
N.D.
N.D.
+0.4
+1.5
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