Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_20495 and PS417_20500 overlap by 30 nucleotidesPS417_20500 and PS417_20505 are separated by 877 nucleotidesPS417_20505 and PS417_20510 are separated by 31 nucleotidesPS417_20510 and PS417_20515 are separated by 203 nucleotides PS417_20495: PS417_20495 - hypothetical protein, at 4,452,194 to 4,452,580 _20495 PS417_20500: PS417_20500 - hypothetical protein, at 4,452,551 to 4,453,000 _20500 PS417_20505: PS417_20505 - hypothetical protein, at 4,453,878 to 4,454,405 _20505 PS417_20510: PS417_20510 - membrane protein, at 4,454,437 to 4,455,303 _20510 PS417_20515: PS417_20515 - FMN-dependent NADH-azoreductase, at 4,455,507 to 4,456,106 _20515
Group Condition PS417_20495 PS417_20500 PS417_20505 PS417_20510 PS417_20515
carbon source octanoate (C) N.D. -0.4 -0.5 -0.7 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. -0.3 -0.6 -0.2 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 -0.3 -0.3 -0.7
stress 1-ethyl-3-methylimidazolium chloride 200 mM N.D. +0.1 -0.7 -0.2 -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.7 -0.3 -0.3 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. -0.6 -0.2 -0.4 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.2 -0.2 -0.2 -0.7
supernatant control Vogels_fungal_media; pH 5.5 N.D. -0.3 -0.6 -0.1 -0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.3 -0.5 +0.1 -0.4
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.4 +0.0 -0.2 -0.5
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) N.D. -0.4 +0.2 -0.1 -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 -0.4 +0.4 -1.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 -0.4 -0.0 -0.7
seeds Growth on radish seeds for 72 hours N.D. -0.1 -0.8 +0.3 +0.0
seeds Growth on radish seeds for 72 hours N.D. -0.3 +0.3 +0.1 -0.6
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. +0.5 +0.1 -0.2 -0.9
soil soil sample 4; outgrowth in LB N.D. +0.2 -0.6 +0.2 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 -0.0 +0.1 -0.8
no stress control TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 N.D. +0.5 -0.2 +0.0 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.6 -0.1 -0.0 -0.6
seeds Growth on radish seeds for 72 hours N.D. +0.5 -0.3 +0.3 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 -0.5 +0.2 -0.2
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x N.D. -0.0 -0.7 +0.2 +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.2 -0.7 +0.2 +0.2
phage P. simiae Grant Run MOI 0.1 N.D. +0.3 -0.6 -0.1 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.0 -0.6 +0.2 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.2 +0.0 -0.3 +0.8
phage P. simiae OR1 MOI 0.1 N.D. +0.5 -0.2 -0.1 +0.6
phage Wcs_1 N.D. +0.9 -0.1 +0.3 +0.3
carbon source L-Valine (C) N.D. +0.3 +0.0 +0.3 +0.8
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