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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_02025 and PS417_02030 are separated by 26 nucleotides
PS417_02030 and PS417_02035 overlap by 1 nucleotides
PS417_02035 and PS417_02040 overlap by 4 nucleotides
PS417_02040 and PS417_02045 overlap by 4 nucleotides
PS417_02025: PS417_02025 - hypothetical protein, at 455,921 to 456,373
_02025
PS417_02030: PS417_02030 - 3-oxoacyl-ACP synthase, at 456,400 to 457,626
_02030
PS417_02035: PS417_02035 - 3-ketoacyl-ACP reductase, at 457,626 to 458,354
_02035
PS417_02040: PS417_02040 - 3-hydroxylacyl-ACP dehydratase, at 458,351 to 458,812
_02040
PS417_02045: PS417_02045 - 3-oxoacyl-ACP synthase, at 458,809 to 459,975
_02045
Group
Condition
PS417
_02025
PS417
_02030
PS417
_02035
PS417
_02040
PS417
_02045
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
+0.1
-0.7
-0.4
-1.9
motility
inner cut, LB soft agar motility assay
+0.3
-0.6
-0.3
-0.9
-0.5
phage
JP1 MOI 1
+0.2
-0.7
+0.2
-0.4
-0.7
phage
P. simiae OR Antrim MOI 10
-0.0
-0.1
+0.5
-0.5
-1.4
phage
JP1 MOI 0.1
+0.3
+0.1
+0.1
-1.0
-1.0
soil
soil sample 5; outgrowth in LB
-0.5
-0.8
-0.2
+0.6
-0.5
phage
P. simiae OR Antrim MOI 10
+0.5
-0.2
-0.1
-0.5
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Cysteine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.1
-0.5
-0.7
-0.4
+0.7
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.6
+0.1
-1.1
-0.2
-0.2
stress
methylglyoxal 0.032 vol%
-0.1
+0.5
+0.6
-0.6
-1.3
seeds
Growth on radish seeds for 72 hours
+0.6
-0.9
+0.3
+0.3
-0.9
seeds
Growth on radish seeds for 72 hours
-0.3
-0.1
+0.8
+0.2
-1.1
phage
P. simiae Grant Run MOI 0.1
+0.5
-0.7
+0.3
+0.3
-0.8
agar plate interaction control
Taped volatile agar plate with no fungus
+0.4
+0.6
-0.4
+0.1
-1.3
root
root sample 6; outgrowth in LB
+0.1
+0.2
+0.6
-0.4
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.2
+0.0
+0.1
+0.8
-1.0
carbon source
L-Valine (C)
+0.4
-0.0
-1.1
+0.1
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
-0.3
-0.8
+0.3
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.0
-0.4
+0.1
+1.1
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.0
+0.4
-0.4
-0.5
+0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.6
+0.7
+0.2
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+0.6
-1.1
+0.5
+0.9
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
-0.0
+0.3
+0.7
-0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.3
+1.0
+0.4
+0.0
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
+0.3
+0.3
-0.1
+0.9
rhizosphere
rhizosphere sample 8; outgrowth in LB
+0.3
-0.6
+0.9
+0.6
+0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.2
+1.3
+0.7
-0.4
-0.1
nitrogen source
Putrescine (N)
-0.3
+0.2
+0.3
+0.6
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.7
+0.2
+0.3
-0.4
+0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
+0.6
+0.5
+0.5
+0.0
+0.6
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