Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_20435 and PS417_20440 are separated by 692 nucleotidesPS417_20440 and PS417_20445 are separated by 140 nucleotidesPS417_20445 and PS417_20450 are separated by 9 nucleotidesPS417_20450 and PS417_20455 are separated by 118 nucleotides PS417_20435: PS417_20435 - membrane protein, at 4,433,818 to 4,437,363 _20435 PS417_20440: PS417_20440 - integrase, at 4,438,056 to 4,439,441 _20440 PS417_20445: PS417_20445 - hypothetical protein, at 4,439,582 to 4,440,427 _20445 PS417_20450: PS417_20450 - hypothetical protein, at 4,440,437 to 4,441,369 _20450 PS417_20455: PS417_20455 - hypothetical protein, at 4,441,488 to 4,442,846 _20455
Group Condition PS417_20435 PS417_20440 PS417_20445 PS417_20450 PS417_20455
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 -0.2 -0.0 -0.6 -0.2
carbon source 2'-Deoxyinosine 5 mM (C) -0.1 -0.3 -0.1 -0.0 -0.5
phage JP1 MOI 10 +0.1 -0.3 -0.1 -0.1 -0.3
stress methylglyoxal 0.032 vol% -0.1 +0.1 +0.3 -0.4 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.0 +0.2 +0.2 -0.6
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -0.1 +0.4 -0.1 +0.0 -0.3
phage P. simiae OR1 MOI 0.1 -0.0 -0.5 +0.1 -0.0 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.0 +0.5 -0.3 -0.1 -0.0
soil soil sample 8; outgrowth in LB +0.2 -0.1 +0.1 +0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.4 +0.1 +0.2 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.2 +0.3 -0.0 +0.2
soil soil sample 4; outgrowth in LB +0.1 +0.2 +0.3 +0.1 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.4 +0.1 -0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 -0.3 +0.1 +0.6 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.1 +0.2 +0.3 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.4 -0.3 -0.0 +0.4
motility inner cut, LB soft agar motility assay +0.2 -0.1 +0.1 +0.2 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 +0.5 +0.0 +0.2 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.4 +0.1 +0.2 +0.1
motility inner cut, LB soft agar motility assay +0.2 +0.3 +0.1 +0.1 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 +0.3 +0.1 +0.3 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 +0.3 +0.1 +0.2 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 +0.3 +0.3 +0.1 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 +0.3 +0.2 +0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.1 +0.2 +0.2 +0.2 +0.4
motility outer cut, LB soft agar motility assay +0.2 +0.2 +0.1 +0.2 +0.4
motility inner cut, LB soft agar motility assay +0.2 +0.4 +0.3 +0.2 +0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.7 +0.1 +0.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +0.8 +0.3 +0.2 +0.1
stress LB_noSalt with Chloride 1100 mM +0.4 +0.5 +0.4 +0.3 +0.3
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