Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_20230 and PS417_20235 are separated by 207 nucleotidesPS417_20235 and PS417_20240 are separated by 65 nucleotidesPS417_20240 and PS417_20245 overlap by 4 nucleotidesPS417_20245 and PS417_20250 overlap by 4 nucleotides PS417_20230: PS417_20230 - hypothetical protein, at 4,395,096 to 4,395,284 _20230 PS417_20235: PS417_20235 - hypothetical protein, at 4,395,492 to 4,396,214 _20235 PS417_20240: PS417_20240 - hypothetical protein, at 4,396,280 to 4,397,146 _20240 PS417_20245: PS417_20245 - copper resistance protein CopD, at 4,397,143 to 4,397,997 _20245 PS417_20250: PS417_20250 - copper resistance protein CopC, at 4,397,994 to 4,398,380 _20250
Group Condition PS417_20230 PS417_20235 PS417_20240 PS417_20245 PS417_20250
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. -0.6 -0.9 -0.3 -0.2
agar plate interaction control Taped volatile agar plate with no fungus N.D. -0.4 -0.2 -0.3 -0.6
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI N.D. -0.6 -0.1 -0.5 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.4 -0.5 -0.5 +0.6
phage JP1 MOI 0.1 N.D. -0.3 -0.3 -0.6 +0.5
stress Nalidixic 0.025 mg/ml N.D. +0.1 +0.5 -0.5 -0.5
seeds Growth on radish seeds for 24 hours N.D. -0.9 +0.4 +0.1 +0.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.3 +0.3 +0.3 -0.6
carbon source 2'-Deoxyinosine 5 mM (C) N.D. +0.4 -0.2 +0.3 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.3 -0.1 -0.9 +0.7
stress 1-ethyl-3-methylimidazolium chloride 200 mM N.D. +0.2 +0.3 +0.4 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.6 +0.4 +0.6 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.5 -0.2 +0.6 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 -0.3 +0.4 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.6 +0.3 +0.4 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 +0.9 -0.3 +0.6
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.5 -0.2 +0.1 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.2 +0.6 +0.6 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.0 +0.5 +1.2 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 -0.2 +1.2 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 +0.4 +0.6 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.1 +0.4 +0.9 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 +0.5 -0.1 +1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 +0.4 +0.4 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.6 +0.3 +0.6 +0.2
motility outer cut, LB soft agar motility assay N.D. +0.5 +0.6 +0.5 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.7 +0.1 +0.4 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 +0.2 +0.6 +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.8 +0.5 +0.9 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 +0.7 +1.2 +0.9
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