Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_20225 and PS417_20230 are separated by 313 nucleotidesPS417_20230 and PS417_20235 are separated by 207 nucleotidesPS417_20235 and PS417_20240 are separated by 65 nucleotidesPS417_20240 and PS417_20245 overlap by 4 nucleotides PS417_20225: PS417_20225 - GntR family transcriptional regulator, at 4,393,946 to 4,394,782 _20225 PS417_20230: PS417_20230 - hypothetical protein, at 4,395,096 to 4,395,284 _20230 PS417_20235: PS417_20235 - hypothetical protein, at 4,395,492 to 4,396,214 _20235 PS417_20240: PS417_20240 - hypothetical protein, at 4,396,280 to 4,397,146 _20240 PS417_20245: PS417_20245 - copper resistance protein CopD, at 4,397,143 to 4,397,997 _20245
Group Condition PS417_20225 PS417_20230 PS417_20235 PS417_20240 PS417_20245
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 N.D. -0.4 -0.5 -0.5
rhizosphere rhizosphere sample 2; outgrowth in LB +0.3 N.D. -0.6 -0.9 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.3 N.D. -0.1 +0.0 -1.1
phage JP1 MOI 1 +0.5 N.D. -0.1 -0.1 -0.8
seeds Growth on radish seeds for 72 hours +0.5 N.D. -0.6 -0.4 -0.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 N.D. -0.6 +0.4 +0.2
seeds Growth on radish seeds for 24 hours -0.0 N.D. -0.9 +0.4 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.4 N.D. +0.3 -0.1 -0.9
seeds Growth on radish seeds for 72 hours -1.1 N.D. +0.1 -0.1 +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.3 N.D. -0.6 -0.3 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 N.D. +0.1 +0.9 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 N.D. +0.5 -0.3 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 N.D. -0.6 +0.4 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 N.D. +0.5 +0.5 -0.3
root root sample 6; outgrowth in LB +0.5 N.D. -0.6 +0.6 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +1.0 N.D. -0.2 +0.4 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.4 N.D. +0.6 +0.3 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 N.D. +0.6 +0.3 +0.4
motility inner cut, LB soft agar motility assay +0.7 N.D. +0.3 +0.4 -0.1
carbon source L-Valine (C) +1.0 N.D. +0.3 +0.3 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 N.D. +0.5 +0.4 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 N.D. +0.7 +0.1 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.3 N.D. +0.2 +0.6 +0.6
stress methylglyoxal 0.032 vol% +1.0 N.D. -0.0 +0.4 +0.4
motility inner cut, LB soft agar motility assay +0.8 N.D. +0.1 +0.5 +0.4
motility outer cut, LB soft agar motility assay +0.3 N.D. +0.5 +0.6 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 N.D. +0.4 -0.2 +1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 N.D. +0.8 +0.5 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 N.D. -0.0 +0.5 +1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 N.D. +0.3 +0.7 +1.2
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PS417_20225
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PS417_20230
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PS417_20240
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PS417_20245
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