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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_19900 and PS417_19905 are separated by 97 nucleotides
PS417_19905 and PS417_19910 overlap by 1 nucleotides
PS417_19910 and PS417_19915 are separated by 64 nucleotides
PS417_19915 and PS417_19920 are separated by 137 nucleotides
PS417_19900: PS417_19900 - phenylalanine 4-monooxygenase, at 4,322,200 to 4,322,991
_19900
PS417_19905: PS417_19905 - pterin-4-alpha-carbinolamine dehydratase, at 4,323,089 to 4,323,445
_19905
PS417_19910: PS417_19910 - aromatic amino acid aminotransferase, at 4,323,445 to 4,324,638
_19910
PS417_19915: PS417_19915 - serine/threonine protein phosphatase, at 4,324,703 to 4,325,512
_19915
PS417_19920: PS417_19920 - AraC family transcriptional regulator, at 4,325,650 to 4,326,399
_19920
Group
Condition
PS417
_19900
PS417
_19905
PS417
_19910
PS417
_19915
PS417
_19920
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.9
N.D.
-0.6
-0.2
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.7
N.D.
-0.2
+0.1
-0.5
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.2
N.D.
-0.5
-0.2
-0.3
soil
soil sample 6; outgrowth in LB
-0.6
N.D.
-0.7
+0.1
+0.0
stress
methylglyoxal 0.032 vol%
+0.1
N.D.
-0.6
-0.2
-0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.1
N.D.
-0.8
-0.1
-0.1
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
+0.2
N.D.
-0.5
-0.1
-0.4
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
N.D.
-0.6
-0.5
+0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.7
N.D.
-0.6
+0.2
+0.2
phage
P. simiae Grant Run MOI 0.1
+0.5
N.D.
-0.8
-0.1
-0.1
phage
JP1 MOI 1
+0.5
N.D.
-0.3
-0.5
-0.3
stress
Gentamicin 0.008 mg/ml
-0.5
N.D.
+0.4
-0.1
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
N.D.
-0.9
-0.0
+0.2
seeds
Growth on radish seeds for 72 hours
+0.2
N.D.
-0.9
+0.3
+0.2
phage
P. simiae ORA MOI 0.1
+0.3
N.D.
-0.5
+0.3
-0.1
soil
soil sample 2; outgrowth in LB
-0.5
N.D.
+0.4
+0.4
-0.2
nophagecontrol
Only library
+0.9
N.D.
-0.4
-0.1
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.3
N.D.
+0.9
-0.0
-0.4
seeds
Growth on radish seeds for 72 hours
+0.4
N.D.
-0.5
-0.0
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
N.D.
+0.1
-0.2
-0.3
supernatant
LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
+0.4
N.D.
+0.4
+0.1
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.1
N.D.
+0.4
-0.2
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
N.D.
-0.3
+0.4
-0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.1
N.D.
+0.6
+0.3
-0.2
soil
soil sample 7; outgrowth in LB
-0.0
N.D.
+0.6
+0.4
-0.2
phage
P. simiae Grant Run MOI 10
+0.4
N.D.
+1.0
-0.0
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
+0.8
-0.0
-0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.7
N.D.
+0.3
-0.1
+0.2
soil
soil sample 8; outgrowth in LB
+0.1
N.D.
+0.7
+0.0
+0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.3
N.D.
+0.6
+0.1
+0.4
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