Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_19900 and PS417_19905 are separated by 97 nucleotidesPS417_19905 and PS417_19910 overlap by 1 nucleotidesPS417_19910 and PS417_19915 are separated by 64 nucleotidesPS417_19915 and PS417_19920 are separated by 137 nucleotides PS417_19900: PS417_19900 - phenylalanine 4-monooxygenase, at 4,322,200 to 4,322,991 _19900 PS417_19905: PS417_19905 - pterin-4-alpha-carbinolamine dehydratase, at 4,323,089 to 4,323,445 _19905 PS417_19910: PS417_19910 - aromatic amino acid aminotransferase, at 4,323,445 to 4,324,638 _19910 PS417_19915: PS417_19915 - serine/threonine protein phosphatase, at 4,324,703 to 4,325,512 _19915 PS417_19920: PS417_19920 - AraC family transcriptional regulator, at 4,325,650 to 4,326,399 _19920
Group Condition PS417_19900 PS417_19905 PS417_19910 PS417_19915 PS417_19920
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.9 N.D. -0.6 -0.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.7 N.D. -0.2 +0.1 -0.5
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.2 N.D. -0.5 -0.2 -0.3
soil soil sample 6; outgrowth in LB -0.6 N.D. -0.7 +0.1 +0.0
stress methylglyoxal 0.032 vol% +0.1 N.D. -0.6 -0.2 -0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 N.D. -0.8 -0.1 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.2 N.D. -0.5 -0.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 N.D. -0.6 -0.5 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.7 N.D. -0.6 +0.2 +0.2
phage P. simiae Grant Run MOI 0.1 +0.5 N.D. -0.8 -0.1 -0.1
phage JP1 MOI 1 +0.5 N.D. -0.3 -0.5 -0.3
stress Gentamicin 0.008 mg/ml -0.5 N.D. +0.4 -0.1 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 N.D. -0.9 -0.0 +0.2
seeds Growth on radish seeds for 72 hours +0.2 N.D. -0.9 +0.3 +0.2
phage P. simiae ORA MOI 0.1 +0.3 N.D. -0.5 +0.3 -0.1
soil soil sample 2; outgrowth in LB -0.5 N.D. +0.4 +0.4 -0.2
nophagecontrol Only library +0.9 N.D. -0.4 -0.1 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. +0.9 -0.0 -0.4
seeds Growth on radish seeds for 72 hours +0.4 N.D. -0.5 -0.0 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 N.D. +0.1 -0.2 -0.3
supernatant LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +0.4 N.D. +0.4 +0.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.1 N.D. +0.4 -0.2 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 N.D. -0.3 +0.4 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.1 N.D. +0.6 +0.3 -0.2
soil soil sample 7; outgrowth in LB -0.0 N.D. +0.6 +0.4 -0.2
phage P. simiae Grant Run MOI 10 +0.4 N.D. +1.0 -0.0 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 N.D. +0.8 -0.0 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.7 N.D. +0.3 -0.1 +0.2
soil soil sample 8; outgrowth in LB +0.1 N.D. +0.7 +0.0 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 N.D. +0.6 +0.1 +0.4
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