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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_19585 and PS417_19590 overlap by 4 nucleotides
PS417_19590 and PS417_19595 are separated by 117 nucleotides
PS417_19595 and PS417_19600 are separated by 73 nucleotides
PS417_19600 and PS417_19605 are separated by 187 nucleotides
PS417_19585: PS417_19585 - acyl-CoA dehydrogenase, at 4,260,043 to 4,261,200
_19585
PS417_19590: PS417_19590 - enoyl-CoA hydratase, at 4,261,197 to 4,261,958
_19590
PS417_19595: PS417_19595 - 3-methylcrotonyl-CoA carboxylase, at 4,262,076 to 4,263,959
_19595
PS417_19600: PS417_19600 - exonuclease, at 4,264,033 to 4,264,575
_19600
PS417_19605: PS417_19605 - hypothetical protein, at 4,264,763 to 4,265,047
_19605
Group
Condition
PS417
_19585
PS417
_19590
PS417
_19595
PS417
_19600
PS417
_19605
phage
P. simiae ORA MOI 10
-0.3
-0.9
-0.9
-1.6
-2.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.0
-0.1
-3.4
-2.0
phage
JP1 MOI 1
-0.5
-1.2
-0.5
-0.7
-2.7
phage
P. simiae Grant Run MOI 10
-0.6
-0.6
-0.8
-2.9
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
+0.2
-0.5
-3.3
-1.7
phage
P. simiae OR Antrim MOI 0.1
+0.0
+0.0
-0.8
-1.6
-2.5
soil
soil sample 5; outgrowth in LB
-0.1
-0.0
-0.2
-3.2
-1.0
phage
JP1 MOI 1
-0.1
-0.4
-0.7
-2.9
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-1.0
-0.1
-2.0
-1.3
phage
P. simiae ORA MOI 0.1
-0.0
+0.1
-0.5
-3.7
-0.2
motility
outer cut, LB soft agar motility assay
-0.2
-0.6
+0.2
-3.4
-0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
+0.1
-0.0
-2.8
-1.4
phage
P. simiae OR Antrim MOI 1
-0.2
+0.1
-0.5
-2.2
-1.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
-0.7
+0.1
-1.9
-2.0
nophagecontrol
Only library
-0.2
-1.1
-0.7
-0.1
-2.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
-0.7
+0.1
-0.7
-2.8
phage
JP1 MOI 10
-0.3
+0.2
-0.7
-1.1
-1.9
phage
P. simiae Grant Run MOI 10
-0.8
-0.5
-0.6
-1.2
-0.7
phage
P. simiae Grant Run MOI 0.1
-0.3
-0.9
-0.7
-1.2
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+0.1
-0.5
-0.1
-2.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.3
+0.2
-1.8
-1.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.4
-0.4
-0.2
-2.4
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.2
-0.6
-0.6
-2.4
+0.6
phage
Wcs_1
+0.1
-0.6
-0.1
-2.8
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.4
-0.4
+0.2
-2.8
stress
Gentamicin 0.008 mg/ml
-0.3
+0.5
-0.3
-2.9
+0.6
phage
P. simiae OR Antrim MOI 10
-0.4
-0.7
-0.4
-0.8
+1.4
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.3
+0.2
-0.2
-1.0
+1.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.7
-0.2
+1.7
-1.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.2
+0.2
+0.5
+4.4
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