Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_19580 and PS417_19585 are separated by 13 nucleotidesPS417_19585 and PS417_19590 overlap by 4 nucleotidesPS417_19590 and PS417_19595 are separated by 117 nucleotidesPS417_19595 and PS417_19600 are separated by 73 nucleotides PS417_19580: PS417_19580 - acetyl-CoA carboxylase, at 4,258,413 to 4,260,029 _19580 PS417_19585: PS417_19585 - acyl-CoA dehydrogenase, at 4,260,043 to 4,261,200 _19585 PS417_19590: PS417_19590 - enoyl-CoA hydratase, at 4,261,197 to 4,261,958 _19590 PS417_19595: PS417_19595 - 3-methylcrotonyl-CoA carboxylase, at 4,262,076 to 4,263,959 _19595 PS417_19600: PS417_19600 - exonuclease, at 4,264,033 to 4,264,575 _19600
Group Condition PS417_19580 PS417_19585 PS417_19590 PS417_19595 PS417_19600
phage P. simiae Grant Run MOI 10 -0.6 -0.6 -0.6 -0.8 -2.9
phage JP1 MOI 1 -0.4 -0.1 -0.4 -0.7 -2.9
phage P. simiae ORA MOI 0.1 -0.3 -0.0 +0.1 -0.5 -3.7
phage P. simiae ORA MOI 10 -0.3 -0.3 -0.9 -0.9 -1.6
rhizosphere rhizosphere sample 2; outgrowth in LB -0.3 -0.2 -0.6 -0.6 -2.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.0 +0.2 -0.5 -3.3
motility outer cut, LB soft agar motility assay +0.4 -0.2 -0.6 +0.2 -3.4
phage P. simiae Grant Run MOI 10 -0.3 -0.8 -0.5 -0.6 -1.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.1 +0.1 -0.0 -2.8
phage Wcs_1 +0.3 +0.1 -0.6 -0.1 -2.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 +0.1 -1.0 -0.1 -2.0
phage P. simiae Grant Run MOI 0.1 +0.0 -0.3 -0.9 -0.7 -1.2
phage P. simiae OR Antrim MOI 1 -0.3 -0.2 +0.1 -0.5 -2.2
phage P. simiae OR Antrim MOI 0.1 -0.0 -0.3 -0.4 -0.2 -2.1
phage JP1 MOI 10 -0.9 -0.3 +0.2 -0.7 -1.1
phage JP1 MOI 1 -0.1 -0.5 -1.2 -0.5 -0.7
soil soil sample 5; outgrowth in LB +0.7 -0.1 -0.0 -0.2 -3.2
carbon source 2'-Deoxyinosine 5 mM (C) -0.5 -0.5 +0.4 +0.1 -2.3
phage P. simiae OR Antrim MOI 0.1 -0.4 +0.0 +0.0 -0.8 -1.6
stress Gentamicin 0.008 mg/ml +0.3 -0.3 +0.5 -0.3 -2.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 +0.4 -0.7 +0.1 -1.9
nophagecontrol Only library -0.8 -0.2 -1.1 -0.7 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.0 -0.1 -0.0 -0.1 -3.4
soil soil sample 3; outgrowth in LB +0.5 -0.2 -0.1 -0.6 -2.0
carbon source L-Valine (C) -0.8 +0.1 -0.0 +0.3 -1.7
stress methylglyoxal 0.032 vol% +0.1 +0.1 +0.2 -0.5 -1.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.3 -0.5 +0.2 -1.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.3 +0.5 -0.3 +0.5 -1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.1 -0.1 +0.4 -0.2 +1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 +0.1 +0.7 -0.2 +1.7
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