Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_19570 and PS417_19575 are separated by 13 nucleotidesPS417_19575 and PS417_19580 are separated by 3 nucleotidesPS417_19580 and PS417_19585 are separated by 13 nucleotidesPS417_19585 and PS417_19590 overlap by 4 nucleotides PS417_19570: PS417_19570 - terpene utilization protein AtuA, at 4,255,745 to 4,257,523 _19570 PS417_19575: PS417_19575 - 2,4-dienoyl-CoA reductase, at 4,257,537 to 4,258,409 _19575 PS417_19580: PS417_19580 - acetyl-CoA carboxylase, at 4,258,413 to 4,260,029 _19580 PS417_19585: PS417_19585 - acyl-CoA dehydrogenase, at 4,260,043 to 4,261,200 _19585 PS417_19590: PS417_19590 - enoyl-CoA hydratase, at 4,261,197 to 4,261,958 _19590
Group Condition PS417_19570 PS417_19575 PS417_19580 PS417_19585 PS417_19590
nophagecontrol Only library -0.2 N.D. -0.8 -0.2 -1.1
phage P. simiae Grant Run MOI 10 -0.4 N.D. -0.6 -0.6 -0.6
phage JP1 MOI 10 -0.5 N.D. -0.4 -0.1 -1.1
phage JP1 MOI 1 -0.2 N.D. -0.1 -0.5 -1.2
phage P. simiae Grant Run MOI 10 -0.4 N.D. -0.3 -0.8 -0.5
phage P. simiae ORA MOI 10 -0.4 N.D. -0.3 -0.3 -0.9
carbon source L-Asparagine (C) -0.1 N.D. -0.2 -0.2 -0.9
soil soil sample 8; outgrowth in LB -0.2 N.D. -0.3 +0.1 -0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 N.D. -0.9 +0.1 -0.6
phage JP1 MOI 10 -0.1 N.D. -0.9 -0.3 +0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 N.D. +0.2 -0.3 -0.7
phage P. simiae OR Antrim MOI 10 -0.3 N.D. +0.3 -0.4 -0.7
phage JP1 MOI 0.1 +0.2 N.D. +0.1 -0.5 -0.8
supernatant control Vogels_fungal_media 0.1X -0.3 N.D. +0.1 +0.4 -1.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 N.D. -0.7 +0.4 -0.7
seeds Growth on radish seeds for 72 hours -0.1 N.D. -0.6 +0.3 -0.5
carbon source 2'-Deoxyinosine 5 mM (C) -0.2 N.D. -0.5 -0.5 +0.4
motility outer cut, LB soft agar motility assay -0.3 N.D. +0.4 -0.2 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. -1.1 -0.1 +0.4
seeds Growth on radish seeds for 72 hours -0.1 N.D. -0.7 +0.3 -0.2
agar plate interaction control Taped volatile agar plate with no fungus +0.4 N.D. +0.0 -0.0 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. +0.4 -0.0 -1.1
phage P. simiae OR Antrim MOI 1 -0.5 N.D. +0.4 -0.4 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 N.D. +0.7 +0.0 -0.7
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 N.D. -0.3 -0.3 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.2 N.D. -0.3 +0.5 -0.3
carbon source 2'-Deoxyinosine 5 mM (C) -0.4 N.D. +0.7 +0.1 -0.3
soil soil sample 7; outgrowth in LB -0.2 N.D. -0.7 +0.2 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 N.D. +0.4 +0.3 -0.5
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 N.D. +0.2 -0.0 +1.2
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