Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_19530 and PS417_19535 overlap by 25 nucleotidesPS417_19535 and PS417_19540 are separated by 71 nucleotidesPS417_19540 and PS417_19545 are separated by 149 nucleotidesPS417_19545 and PS417_19550 are separated by 53 nucleotides PS417_19530: PS417_19530 - peroxiredoxin, at 4,234,980 to 4,235,831 _19530 PS417_19535: PS417_19535 - dihydroneopterin aldolase, at 4,235,807 to 4,236,568 _19535 PS417_19540: PS417_19540 - hypothetical protein, at 4,236,640 to 4,239,351 _19540 PS417_19545: PS417_19545 - peptide synthase, at 4,239,501 to 4,252,382 _19545 PS417_19550: PS417_19550 - thioesterase, at 4,252,436 to 4,253,188 _19550
Group Condition PS417_19530 PS417_19535 PS417_19540 PS417_19545 PS417_19550
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.4 -0.0 +0.0 -1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.2 +0.1 +0.0 -0.5
phage JP1 MOI 0.1 N.D. -0.8 +0.1 -0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.5 -0.1 -0.1 -0.5
carbon source L-Histidine (C) N.D. -0.6 -0.0 -0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.6 -0.0 -0.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.2 +0.0 -0.1 -0.8
phage JP1 MOI 10 N.D. -0.6 +0.1 +0.0 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.4 +0.3 +0.0 -0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.8 -0.0 +0.2 -0.3
soil soil sample 2; outgrowth in LB N.D. -0.7 -0.2 +0.1 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 +0.2 +0.1 -0.6
phage JP1 MOI 1 N.D. -0.6 -0.0 -0.2 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 +0.1 +0.3 -0.4
phage P. simiae ORA MOI 0.1 N.D. +0.2 +0.1 +0.1 -0.8
seeds Growth on radish seeds for 24 hours N.D. +0.4 +0.1 +0.0 -0.6
motility outer cut, LB soft agar motility assay N.D. +0.2 +0.0 +0.3 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.7 +0.1 +0.1 +0.7
seeds Growth on radish seeds for 72 hours N.D. +0.4 +0.3 +0.0 -0.5
phage P. simiae Grant Run MOI 0.1 N.D. +0.5 +0.2 -0.1 -0.5
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. -0.3 -0.0 +0.2 +0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.1 -0.2 +0.2 +0.7
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +1.2 -0.1 -0.6 +0.2
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +1.5 -0.0 -0.3 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.0 +0.3 +0.0 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.0 +0.2 +0.2 +0.8
solid stress Fraxetin 0.5 mM; solid stress N.D. +0.4 +0.0 +0.3 +0.4
motility inner cut, LB soft agar motility assay N.D. +0.4 +0.1 +0.3 +0.4
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. -0.1 -0.0 +1.5 +1.8
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. -0.0 +0.1 +2.2 +2.5
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