Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_19475 and PS417_19480 are separated by 171 nucleotidesPS417_19480 and PS417_19490 are separated by 413 nucleotidesPS417_19490 and PS417_19495 are separated by 17 nucleotidesPS417_19495 and PS417_19500 are separated by 125 nucleotides PS417_19475: PS417_19475 - pseudouridine synthase, at 4,222,185 to 4,222,820 _19475 PS417_19480: PS417_19480 - aminopeptidase, at 4,222,992 to 4,224,281 _19480 PS417_19490: PS417_19490 - mechanosensitive ion channel protein, at 4,224,695 to 4,226,860 _19490 PS417_19495: PS417_19495 - N-acyl-L-amino acid amidohydrolase, at 4,226,878 to 4,228,224 _19495 PS417_19500: PS417_19500 - antibiotic synthesis protein MbtH, at 4,228,350 to 4,228,574 _19500
Group Condition PS417_19475 PS417_19480 PS417_19490 PS417_19495 PS417_19500
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.7 -0.2 +0.2 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -1.2 +0.1 -0.2 -0.5
phage P. simiae Grant Run MOI 10 -0.8 -0.6 -0.7 -0.1 +0.4
agar plate interaction control Taped volatile agar plate with no fungus +0.3 -0.5 -0.4 -0.1 -0.7
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -0.3 +0.1 -0.6 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.8 +0.4 +0.2 -1.2
stress Cisplatin 0.01 mg/ml +0.5 -0.8 -0.1 -0.4 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.2 -0.4 +0.7 -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -1.0 -0.2 +0.6 -0.4
stress Doxycycline hyclate 0.001 mg/ml -0.6 +0.6 -0.1 -0.0 -1.0
phage Wcs_1 -0.5 -1.0 -0.1 -0.1 +0.6
solid stress Fraxetin 3 mM; solid stress -0.5 -1.0 +0.3 +0.0 +0.4
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 -0.3 -0.4 -0.1 +0.8
solid stress Fraxetin 2 mM; solid stress -0.6 -1.1 +0.5 +0.2 +0.5
rhizosphere rhizosphere sample 1; outgrowth in LB -0.0 -0.5 -0.4 -0.2 +1.0
stress methylglyoxal 0.032 vol% -0.8 -0.4 +1.0 -0.4 +0.5
solid stress Fraxetin 3 mM; solid stress -0.1 -1.2 +0.6 +0.3 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.9 -0.3 +0.2 -0.0 +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -0.4 -0.9 +0.5 +0.6
stress Gentamicin 0.008 mg/ml -1.3 +1.1 +0.3 +0.2 -0.3
phage P. simiae OR Antrim MOI 0.1 -0.4 -0.1 -0.4 +0.0 +1.2
phage P. simiae OR1 MOI 0.1 +0.5 -0.5 +0.3 -0.3 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.5 +0.8 +0.3 +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 -0.2 +0.9 +0.3 +0.4
soil soil sample 7; outgrowth in LB +0.6 -0.5 -0.1 +0.2 +0.8
seeds Growth on radish seeds for 24 hours +0.4 -0.6 +0.5 +0.2 +1.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.2 +0.9 +0.2 +1.1
seeds Growth on radish seeds for 72 hours +0.2 +0.4 -0.2 +0.8 +1.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.2 +0.3 +0.2 +0.3 +1.5
stress 1-ethyl-3-methylimidazolium acetate 35 mM -0.4 -0.7 +3.3 +0.6 +0.4
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