Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_19440 and PS417_19445 are separated by 436 nucleotidesPS417_19445 and PS417_19450 are separated by 31 nucleotidesPS417_19450 and PS417_19455 are separated by 30 nucleotidesPS417_19455 and PS417_19460 are separated by 119 nucleotides PS417_19440: PS417_19440 - purine permease, at 4,215,295 to 4,216,641 _19440 PS417_19445: PS417_19445 - membrane protein, at 4,217,078 to 4,217,863 _19445 PS417_19450: PS417_19450 - Patatin, at 4,217,895 to 4,219,028 _19450 PS417_19455: PS417_19455 - lipid A biosynthesis lauroyl acyltransferase, at 4,219,059 to 4,219,994 _19455 PS417_19460: PS417_19460 - septum formation inhibitor, at 4,220,114 to 4,220,851 _19460
Group Condition PS417_19440 PS417_19445 PS417_19450 PS417_19455 PS417_19460
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) -0.6 -0.1 -0.6 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.3 -0.7 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.4 -0.4 -0.2 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 -0.1 -1.0 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.5 -0.1 -0.5 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.7 -0.2 -0.1 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.6 -0.0 -0.4 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.1 -0.7 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 -0.2 -0.8 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.2 +0.0 -0.7 N.D. N.D.
soil soil sample 3; outgrowth in LB -0.7 -0.1 +0.3 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.0 -0.7 N.D. N.D.
phage JP1 MOI 10 -0.6 -0.1 +0.3 N.D. N.D.
soil soil sample 5; outgrowth in LB -0.6 -0.3 +0.4 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.5 +0.1 -0.6 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.0 +0.6 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 -0.4 +0.6 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +0.2 -0.5 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.0 -0.2 -0.5 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 -0.1 +0.8 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 -0.1 -0.4 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.2 +1.1 N.D. N.D.
stress Neomycin 0.04 mg/ml -0.2 +0.3 +0.5 N.D. N.D.
phage JP1 MOI 1 +0.6 +0.2 -0.2 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.5 +0.4 N.D. N.D.
phage P. simiae OR Antrim MOI 0.1 +0.8 +0.1 -0.2 N.D. N.D.
rhizosphere rhizosphere sample 2; outgrowth in LB +0.6 -0.1 +0.5 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +0.4 -0.0 N.D. N.D.
seeds Growth on radish seeds for 72 hours +0.6 +0.3 +0.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 +0.1 +0.3 N.D. N.D.
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