Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_19375 and PS417_19380 are separated by 104 nucleotidesPS417_19380 and PS417_19385 are separated by 10 nucleotidesPS417_19385 and PS417_19390 are separated by 81 nucleotidesPS417_19390 and PS417_19395 are separated by 175 nucleotides PS417_19375: PS417_19375 - LysR family transcriptional regulator, at 4,199,066 to 4,199,962 _19375 PS417_19380: PS417_19380 - glutathione S-transferase, at 4,200,067 to 4,200,669 _19380 PS417_19385: PS417_19385 - GntR family transcriptional regulator, at 4,200,680 to 4,201,846 _19385 PS417_19390: PS417_19390 - MarR family transcriptional regulator, at 4,201,928 to 4,202,359 _19390 PS417_19395: PS417_19395 - guanine permease, at 4,202,535 to 4,203,881 _19395
Group Condition PS417_19375 PS417_19380 PS417_19385 PS417_19390 PS417_19395
carbon source Inosine (C) +0.1 -0.3 -0.1 N.D. -1.9
stress methylglyoxal 0.032 vol% -0.6 -1.8 -0.1 N.D. +0.5
carbon source 2'-Deoxyinosine 5 mM (C) +0.3 +0.3 -0.0 N.D. -2.6
carbon source Inosine (C) +0.3 -0.0 -0.2 N.D. -1.9
carbon source 2'-Deoxyinosine 5 mM (C) +0.4 +0.2 +0.1 N.D. -2.2
phage P. simiae Grant Run MOI 0.1 -0.5 -0.1 -0.6 N.D. -0.1
phage JP1 MOI 10 -0.2 -0.2 -0.1 N.D. -0.6
phage P. simiae ORA MOI 10 -0.7 +0.1 -0.3 N.D. -0.1
phage P. simiae Grant Run MOI 10 -0.6 -0.2 -0.3 N.D. +0.1
phage P. simiae ORA MOI 0.1 -0.1 -0.5 -0.4 N.D. +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 -0.5 -0.1 N.D. -0.4
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X -0.5 +0.2 -0.4 N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.1 -0.5 N.D. +0.3
solid stress Fraxetin 3 mM; solid stress +0.4 -0.5 -0.2 N.D. -0.0
phage P. simiae OR Antrim MOI 1 -0.5 +0.1 -0.1 N.D. +0.5
agar plate interaction control Taped volatile agar plate with no fungus +0.0 -0.3 +0.6 N.D. -0.3
phage P. simiae OR1 MOI 0.1 -0.4 +0.2 -0.0 N.D. +0.5
rhizosphere rhizosphere sample 1; outgrowth in LB +0.2 +0.4 -0.5 N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.6 +0.1 -0.3 N.D. -0.1
phage P. simiae ORA MOI 0.1 +0.3 -0.2 -0.3 N.D. +0.5
stress Gentamicin 0.008 mg/ml -0.3 +0.5 +0.3 N.D. -0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.9 -0.1 -0.1 N.D. -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.1 +0.3 -0.2 N.D. +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.1 +0.4 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 +0.9 -0.1 N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 +0.1 -0.1 N.D. +0.3
carbon source L-Valine (C) -0.1 +0.5 +0.5 N.D. +0.4
root root sample 6; outgrowth in LB +0.3 +0.5 +0.3 N.D. +0.3
carbon source L-Valine (C) +0.8 +0.4 +0.0 N.D. +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.7 +0.4 N.D. +0.3
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PS417_19375
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PS417_19380
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PS417_19390
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PS417_19395
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