Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_19340 and PS417_19345 are separated by 75 nucleotidesPS417_19345 and PS417_19350 are separated by 78 nucleotidesPS417_19350 and PS417_19355 are separated by 101 nucleotidesPS417_19355 and PS417_19360 are separated by 22 nucleotides PS417_19340: PS417_19340 - hypothetical protein, at 4,193,945 to 4,194,265 _19340 PS417_19345: PS417_19345 - DNA mismatch repair protein MutS, at 4,194,341 to 4,194,898 _19345 PS417_19350: PS417_19350 - GTP cyclohydrolase, at 4,194,977 to 4,195,522 _19350 PS417_19355: PS417_19355 - glutaredoxin, at 4,195,624 to 4,195,995 _19355 PS417_19360: PS417_19360 - hypothetical protein, at 4,196,018 to 4,196,338 _19360
Group Condition PS417_19340 PS417_19345 PS417_19350 PS417_19355 PS417_19360
motility outer cut, LB soft agar motility assay N.D. N.D. -4.0 -0.0 +0.3
motility outer cut, LB soft agar motility assay N.D. N.D. -3.3 -0.3 -0.1
motility inner cut, LB soft agar motility assay N.D. N.D. -3.7 +0.2 -0.2
liquid stress 80% Methanol 3 mM; liquid stress N.D. N.D. -2.8 -0.2 -0.5
phage P. simiae ORA MOI 0.1 N.D. N.D. -3.0 +0.0 -0.5
soil soil sample 8; outgrowth in LB N.D. N.D. -2.9 -0.0 -0.5
solid stress Fraxetin 3 mM; solid stress N.D. N.D. -3.2 +0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -2.0 -0.6 -0.6
phage P. simiae ORA MOI 10 N.D. N.D. -2.0 -0.9 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -2.2 -0.3 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.8 +0.1 -0.3
phage P. simiae ORA MOI 1 N.D. N.D. -3.3 -0.6 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -2.0 -0.9 -0.1
motility inner cut, LB soft agar motility assay N.D. N.D. -3.2 +0.3 -0.1
stress Cisplatin 0.01 mg/ml N.D. N.D. -2.5 -0.2 -0.1
soil soil sample 4; outgrowth in LB N.D. N.D. -1.8 +0.3 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -3.1 -0.3 +0.8
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. -2.6 -0.1 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -2.5 -0.3 +0.4
solid stress 80% Methanol 2 mM; solid stress N.D. N.D. -2.7 +0.3 +0.1
solid stress 80% Methanol 3 mM; solid stress N.D. N.D. -2.4 +0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -2.0 +1.0 -1.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -1.9 +0.4 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.6 -0.2 +0.7
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. N.D. -2.3 +0.5 +0.2
solid stress 80% Methanol 3 mM; solid stress N.D. N.D. -2.3 -0.0 +0.7
solid stress Fraxetin 2 mM; solid stress N.D. N.D. -2.2 +0.3 +0.5
solid stress 80% Methanol 2 mM; solid stress N.D. N.D. -2.1 +0.7 +0.3
phage JP1 MOI 10 N.D. N.D. -1.9 +0.3 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.8 +0.0 +2.4
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