Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_19240 and PS417_19245 are separated by 59 nucleotidesPS417_19245 and PS417_19255 are separated by 341 nucleotidesPS417_19255 and PS417_19265 are separated by 545 nucleotidesPS417_19265 and PS417_19270 are separated by 295 nucleotides PS417_19240: PS417_19240 - peptidase M42, at 4,176,284 to 4,177,468 _19240 PS417_19245: PS417_19245 - hypothetical protein, at 4,177,528 to 4,177,755 _19245 PS417_19255: PS417_19255 - short-chain dehydrogenase, at 4,178,097 to 4,178,912 _19255 PS417_19265: PS417_19265 - membrane protein, at 4,179,458 to 4,181,545 _19265 PS417_19270: PS417_19270 - hypothetical protein, at 4,181,841 to 4,182,032 _19270
Group Condition PS417_19240 PS417_19245 PS417_19255 PS417_19265 PS417_19270
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 N.D. -0.6 -0.2 -1.1
seeds Growth on radish seeds for 24 hours -1.0 N.D. -0.6 -0.1 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 N.D. -0.5 -0.9 -0.3
seeds Growth on radish seeds for 72 hours -0.9 N.D. -0.3 -0.9 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 N.D. -0.2 -0.6 -1.1
stress Doxycycline hyclate 0.001 mg/ml +0.1 N.D. -0.5 -0.4 -1.1
phage JP1 MOI 10 -0.1 N.D. -0.0 +0.3 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.6 N.D. +0.6 -0.3 -0.6
soil soil sample 7; outgrowth in LB -0.7 N.D. +0.6 -0.2 -0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.6 N.D. -0.3 +1.8 -0.7
phage JP1 MOI 0.1 -0.7 N.D. -0.1 +0.6 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 N.D. +0.6 -1.0 +0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.0 N.D. -0.0 +1.6 -0.7
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.6 N.D. +0.2 -0.2 -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 N.D. +0.3 +1.2 -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.3 N.D. +0.9 +1.4 -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +1.0 N.D. -0.1 -0.2 +0.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.3 N.D. +0.6 +1.0 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.3 N.D. +0.5 +1.7 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +1.5 N.D. -0.3 -0.6 +1.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.4 N.D. +0.5 +1.7 -0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.4 N.D. +0.5 +1.2 -0.4
motility inner cut, LB soft agar motility assay +0.7 N.D. +0.4 +0.2 +0.8
phage JP1 MOI 1 +0.2 N.D. -0.3 +0.2 +2.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 N.D. -0.2 -0.5 +3.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 N.D. +0.3 +1.0 +0.6
phage JP1 MOI 10 +0.6 N.D. +0.3 -0.0 +1.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.0 N.D. -0.2 +0.4 +1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +2.9 N.D. +0.2 -0.1 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.4 N.D. -0.2 +0.2 +2.0
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