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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_19210 and PS417_19215 are separated by 91 nucleotides
PS417_19215 and PS417_19220 are separated by 102 nucleotides
PS417_19220 and PS417_19225 are separated by 212 nucleotides
PS417_19225 and PS417_19230 are separated by 134 nucleotides
PS417_19210: PS417_19210 - acetyl-CoA acetyltransferase, at 4,162,653 to 4,163,837
_19210
PS417_19215: PS417_19215 - polyphosphate kinase, at 4,163,929 to 4,165,455
_19215
PS417_19220: PS417_19220 - hypothetical protein, at 4,165,558 to 4,170,402
_19220
PS417_19225: PS417_19225 - FAD-dependent cmnm(5)s(2)U34 oxidoreductase, at 4,170,615 to 4,172,615
_19225
PS417_19230: PS417_19230 - asparagine synthase, at 4,172,750 to 4,174,522
_19230
Group
Condition
PS417
_19210
PS417
_19215
PS417
_19220
PS417
_19225
PS417
_19230
phage
JP1 MOI 1
-1.3
-0.4
+0.0
-0.3
-0.2
stress
LB_noSalt with Chloride 1100 mM
-0.1
-0.3
+0.0
-0.4
-1.3
phage
JP1 MOI 10
-0.3
-1.1
-0.2
-0.3
-0.0
phage
P. simiae OR Antrim MOI 10
-0.2
-0.7
+0.2
-0.3
-0.3
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
-0.5
+0.1
+0.0
-0.3
-0.6
stress
Gentamicin 0.008 mg/ml
+0.0
-1.2
+0.0
-0.3
+0.2
phage
P. simiae Grant Run MOI 10
-0.2
-0.8
+0.1
+0.2
-0.4
stress
LB_noSalt with Chloride 1100 mM
+0.1
+0.3
-0.1
-0.5
-0.7
solid stress
Fraxetin 3 mM; solid stress
-0.1
-0.6
+0.0
-0.6
+0.4
phage
P. simiae OR Antrim MOI 0.1
+0.1
-0.8
+0.2
-0.3
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.5
+0.4
-0.1
-0.6
-0.5
carbon source
L-Valine (C)
+0.4
-0.9
-0.0
+0.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.8
-0.6
-0.0
-0.7
+0.3
soil
soil sample 8; outgrowth in LB
+0.2
-0.6
+0.3
-0.6
+0.4
root
root sample 6; outgrowth in LB
+0.5
-0.7
+0.0
-0.4
+0.3
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.1
+0.4
-0.1
-0.6
+0.2
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
-0.3
+0.8
-0.1
-0.2
-0.3
phage
P. simiae ORA MOI 0.1
-0.4
-0.5
+0.3
+0.3
+0.3
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.4
+0.1
+0.1
-0.7
+0.3
phage
JP1 MOI 1
-0.4
+0.6
-0.1
-0.3
+0.4
carbon source
2'-Deoxyinosine 5 mM (C)
+0.2
+0.8
-0.0
-0.1
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.5
+0.2
-0.3
+0.6
agar plate interaction control
Taped volatile agar plate with no fungus
+0.2
+0.8
+0.1
-1.0
+0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.0
+1.2
-0.2
-0.6
-0.0
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+1.3
-0.1
-0.4
-0.0
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.1
+0.9
+0.0
-0.4
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.6
+0.3
+0.4
+0.2
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
+0.3
+0.6
-0.1
+0.4
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+1.0
+0.3
+0.2
+0.4
seeds
Growth on radish seeds for 72 hours
+0.5
+1.1
+0.1
+0.4
+0.3
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