Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_19210 and PS417_19215 are separated by 91 nucleotidesPS417_19215 and PS417_19220 are separated by 102 nucleotidesPS417_19220 and PS417_19225 are separated by 212 nucleotidesPS417_19225 and PS417_19230 are separated by 134 nucleotides PS417_19210: PS417_19210 - acetyl-CoA acetyltransferase, at 4,162,653 to 4,163,837 _19210 PS417_19215: PS417_19215 - polyphosphate kinase, at 4,163,929 to 4,165,455 _19215 PS417_19220: PS417_19220 - hypothetical protein, at 4,165,558 to 4,170,402 _19220 PS417_19225: PS417_19225 - FAD-dependent cmnm(5)s(2)U34 oxidoreductase, at 4,170,615 to 4,172,615 _19225 PS417_19230: PS417_19230 - asparagine synthase, at 4,172,750 to 4,174,522 _19230
Group Condition PS417_19210 PS417_19215 PS417_19220 PS417_19225 PS417_19230
phage JP1 MOI 1 -1.3 -0.4 +0.0 -0.3 -0.2
stress LB_noSalt with Chloride 1100 mM -0.1 -0.3 +0.0 -0.4 -1.3
phage JP1 MOI 10 -0.3 -1.1 -0.2 -0.3 -0.0
phage P. simiae OR Antrim MOI 10 -0.2 -0.7 +0.2 -0.3 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.5 +0.1 +0.0 -0.3 -0.6
stress Gentamicin 0.008 mg/ml +0.0 -1.2 +0.0 -0.3 +0.2
phage P. simiae Grant Run MOI 10 -0.2 -0.8 +0.1 +0.2 -0.4
stress LB_noSalt with Chloride 1100 mM +0.1 +0.3 -0.1 -0.5 -0.7
solid stress Fraxetin 3 mM; solid stress -0.1 -0.6 +0.0 -0.6 +0.4
phage P. simiae OR Antrim MOI 0.1 +0.1 -0.8 +0.2 -0.3 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.5 +0.4 -0.1 -0.6 -0.5
carbon source L-Valine (C) +0.4 -0.9 -0.0 +0.1 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 -0.6 -0.0 -0.7 +0.3
soil soil sample 8; outgrowth in LB +0.2 -0.6 +0.3 -0.6 +0.4
root root sample 6; outgrowth in LB +0.5 -0.7 +0.0 -0.4 +0.3
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.1 +0.4 -0.1 -0.6 +0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.3 +0.8 -0.1 -0.2 -0.3
phage P. simiae ORA MOI 0.1 -0.4 -0.5 +0.3 +0.3 +0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.1 +0.1 -0.7 +0.3
phage JP1 MOI 1 -0.4 +0.6 -0.1 -0.3 +0.4
carbon source 2'-Deoxyinosine 5 mM (C) +0.2 +0.8 -0.0 -0.1 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.5 +0.2 -0.3 +0.6
agar plate interaction control Taped volatile agar plate with no fungus +0.2 +0.8 +0.1 -1.0 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.0 +1.2 -0.2 -0.6 -0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +1.3 -0.1 -0.4 -0.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 +0.9 +0.0 -0.4 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.6 +0.3 +0.4 +0.2
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) +0.3 +0.6 -0.1 +0.4 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +1.0 +0.3 +0.2 +0.4
seeds Growth on radish seeds for 72 hours +0.5 +1.1 +0.1 +0.4 +0.3
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