Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_18875 and PS417_18880 are separated by 39 nucleotidesPS417_18880 and PS417_18885 overlap by 4 nucleotidesPS417_18885 and PS417_18890 are separated by 339 nucleotidesPS417_18890 and PS417_18895 are separated by 19 nucleotides PS417_18875: PS417_18875 - hypothetical protein, at 4,088,299 to 4,088,589 _18875 PS417_18880: PS417_18880 - SAM-dependent methyltransferase, at 4,088,629 to 4,089,324 _18880 PS417_18885: PS417_18885 - GntR family transcriptional regulator, at 4,089,321 to 4,090,040 _18885 PS417_18890: PS417_18890 - MFS transporter, at 4,090,380 to 4,091,741 _18890 PS417_18895: PS417_18895 - glucarate dehydratase, at 4,091,761 to 4,093,086 _18895
Group Condition PS417_18875 PS417_18880 PS417_18885 PS417_18890 PS417_18895
phage JP1 MOI 0.1 N.D. +0.0 -2.4 -0.0 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.2 -1.2 +0.1 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.5 -1.5 +0.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.2 -1.5 -0.0 +0.1
phage P. simiae OR Antrim MOI 1 N.D. -0.1 -1.2 +0.0 -0.4
nophagecontrol Only library N.D. -0.2 -1.5 -0.1 +0.2
motility inner cut, LB soft agar motility assay N.D. -0.5 -0.8 +0.1 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 -1.9 +0.2 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 -1.1 +0.2 -0.2
motility inner cut, LB soft agar motility assay N.D. -0.2 -1.4 +0.0 +0.2
r2a control with 0.2x vogels R2A control with 0.2X Vogels N.D. -0.1 -1.1 -0.2 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 -1.0 -0.2 +0.3
motility outer cut, LB soft agar motility assay N.D. -0.1 -1.3 +0.1 +0.1
carbon source octanoate (C) N.D. +0.2 -1.2 -0.0 -0.1
carbon source L-Valine (C) N.D. +0.2 -1.2 +0.2 -0.2
stress Gentamicin 0.008 mg/ml N.D. +0.3 -1.4 -0.0 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.6 -0.7 +0.4 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.1 -1.2 +0.2 +0.2
seeds Growth on radish seeds for 72 hours N.D. -0.2 -0.2 -0.7 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 -0.7 +0.5 +0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.4 +0.9 -0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.1 +0.7 +0.3 -0.4
stress Choline chloride 300 mM N.D. +0.3 +0.9 -0.1 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.2 +1.1 +0.1 -0.2
carbon source 2-Deoxy-D-Ribose 10 mM (C) N.D. -0.3 +1.3 +0.4 -0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.1 +1.5 -0.3 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 +0.7 +0.8 -0.0
carbon source D-Mannose (C) N.D. +0.3 +1.2 +0.1 +0.1
carbon source D-Mannose (C) N.D. +0.2 +1.8 +0.0 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.7 +1.4 +0.1 +0.0
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