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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_18775 and PS417_18780 are separated by 8 nucleotides
PS417_18780 and PS417_18790 are separated by 180 nucleotides
PS417_18790 and PS417_18795 are separated by 112 nucleotides
PS417_18795 and PS417_18800 are separated by 53 nucleotides
PS417_18775: PS417_18775 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase, at 4,072,091 to 4,073,119
_18775
PS417_18780: PS417_18780 - flavin reductase, at 4,073,128 to 4,073,637
_18780
PS417_18790: PS417_18790 - amino acid transporter LysE, at 4,073,818 to 4,074,426
_18790
PS417_18795: PS417_18795 - LysR family transcriptional regulator, at 4,074,539 to 4,075,402
_18795
PS417_18800: PS417_18800 - hypothetical protein, at 4,075,456 to 4,075,818
_18800
Group
Condition
PS417
_18775
PS417
_18780
PS417
_18790
PS417
_18795
PS417
_18800
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-1.5
+0.0
-0.2
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-1.8
-0.1
-0.5
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.7
-0.2
-0.2
-0.2
-1.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
-2.0
-0.1
-0.6
+0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-1.9
-0.2
+0.1
-0.2
carbon source
Carbon source D-Mannitol 10 mM
-0.4
-1.7
+0.3
+0.2
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.2
-1.6
+0.0
-0.3
-0.3
phage
JP1 MOI 1
+0.5
-0.7
-0.2
-0.8
-0.4
soil
soil sample 4; outgrowth in LB
+0.4
-1.1
+0.0
-0.1
-0.7
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
-0.7
-0.8
+0.5
+0.2
+0.1
phage
P. simiae OR Antrim MOI 1
+0.4
-1.8
+0.5
+0.0
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
+0.8
-0.4
-0.3
-1.0
carbon source
L-Valine (C)
-0.8
+0.3
-0.3
+0.8
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
-0.5
-0.1
+0.1
+1.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.3
-1.4
+0.4
+0.3
+1.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+1.5
-1.5
-0.3
-0.1
+0.7
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.7
-0.8
+0.4
-0.3
+0.1
phage
P. simiae ORA MOI 0.1
-0.4
+1.1
+0.3
-0.3
-0.5
agar plate interaction control
Taped volatile agar plate with no fungus
+0.1
+1.4
-0.1
-0.1
-1.0
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.9
-0.7
+0.4
+0.3
-0.4
stress
methylglyoxal 0.032 vol%
+0.6
+0.7
+0.4
-0.2
-1.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.8
-1.0
+0.4
+0.2
+1.8
rhizosphere
rhizosphere sample 8; outgrowth in LB
+0.7
+0.8
-0.6
+0.2
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
+1.1
+0.3
-0.1
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.3
-0.5
-0.1
+1.2
+0.3
phage
P. simiae OR1 MOI 0.1
+0.5
+0.8
-0.4
-0.2
+0.6
seeds
Growth on radish seeds for 24 hours
-0.5
+0.8
-0.2
+0.4
+0.7
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.4
+0.5
+0.2
-0.3
+1.1
seeds
Growth on radish seeds for 72 hours
+0.6
-0.9
+0.6
+0.3
+1.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.8
+1.2
-0.5
+0.5
+0.2
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