Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_18740 and PS417_18745 overlap by 4 nucleotidesPS417_18745 and PS417_18750 are separated by 50 nucleotidesPS417_18750 and PS417_18755 overlap by 1 nucleotidesPS417_18755 and PS417_18760 are separated by 130 nucleotides PS417_18740: PS417_18740 - acetyltransferase, at 4,067,644 to 4,068,141 _18740 PS417_18745: PS417_18745 - hypothetical protein, at 4,068,138 to 4,068,362 _18745 PS417_18750: PS417_18750 - esterase, at 4,068,413 to 4,069,339 _18750 PS417_18755: PS417_18755 - CrtK, at 4,069,339 to 4,069,779 _18755 PS417_18760: PS417_18760 - hypothetical protein, at 4,069,910 to 4,070,479 _18760
Group Condition PS417_18740 PS417_18745 PS417_18750 PS417_18755 PS417_18760
phage P. simiae OR Antrim MOI 10 +0.1 N.D. +0.6 -2.5 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.9 N.D. +0.4 -2.7 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 N.D. -0.2 -0.4 -2.7
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -0.3 N.D. -0.3 -0.8 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 N.D. +0.3 -0.4 -1.7
phage P. simiae ORA MOI 0.1 -0.0 N.D. +0.2 -0.9 -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.3 N.D. +0.3 -0.9 -1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 N.D. +0.1 -1.1 +0.5
root root sample 6; outgrowth in LB -1.2 N.D. -0.0 +0.6 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 N.D. +1.0 -0.8 -0.4
soil soil sample 3; outgrowth in LB -0.6 N.D. -0.0 +0.9 -1.1
seeds Growth on radish seeds for 72 hours -0.6 N.D. -0.3 +0.7 -0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.0 N.D. +0.5 +0.2 -1.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 N.D. +0.2 -0.9 +0.9
phage Wcs_1 -0.8 N.D. -0.2 +0.8 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.5 N.D. +0.3 -0.5 -0.8
agar plate interaction control Taped volatile agar plate with no fungus +0.1 N.D. -0.5 -0.9 +1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +1.2 N.D. -0.1 -0.9 -0.4
carbon source D-Galacturonic Acid (C) +0.1 N.D. +0.3 -1.0 +0.7
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 N.D. +0.3 +1.0 -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.7 N.D. +0.4 -0.3 -0.7
carbon source L-Valine (C) +0.9 N.D. -0.3 -0.8 +0.3
seeds Growth on radish seeds for 72 hours -0.7 N.D. -0.5 +0.5 +1.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 N.D. -0.3 +2.1 -0.9
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -1.1 N.D. +0.4 +1.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 N.D. -0.5 +1.3 -0.4
phage P. simiae OR1 MOI 0.1 -0.3 N.D. +0.1 +1.8 -0.4
phage JP1 MOI 10 +0.2 N.D. +0.0 +1.5 -0.5
seeds Growth on radish seeds for 24 hours -0.7 N.D. -0.1 +1.1 +1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 N.D. +0.3 +0.2 +1.4
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