Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_18725 and PS417_18730 overlap by 4 nucleotidesPS417_18730 and PS417_18735 overlap by 4 nucleotidesPS417_18735 and PS417_18740 are separated by 21 nucleotidesPS417_18740 and PS417_18745 overlap by 4 nucleotides PS417_18725: PS417_18725 - ABC transporter substrate-binding protein, at 4,064,850 to 4,065,860 _18725 PS417_18730: PS417_18730 - ABC transporter permease, at 4,065,857 to 4,066,861 _18730 PS417_18735: PS417_18735 - histidinol phosphatase, at 4,066,858 to 4,067,622 _18735 PS417_18740: PS417_18740 - acetyltransferase, at 4,067,644 to 4,068,141 _18740 PS417_18745: PS417_18745 - hypothetical protein, at 4,068,138 to 4,068,362 _18745
Group Condition PS417_18725 PS417_18730 PS417_18735 PS417_18740 PS417_18745
phage JP1 MOI 1 -0.4 -1.5 N.D. -0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.9 N.D. -0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.2 -1.1 N.D. -0.5 N.D.
phage P. simiae ORA MOI 0.1 -0.4 -1.2 N.D. -0.2 N.D.
phage JP1 MOI 10 -0.5 -0.9 N.D. -0.3 N.D.
carbon source 2'-Deoxyinosine 5 mM (C) -0.1 -0.8 N.D. -0.7 N.D.
root root sample 6; outgrowth in LB +0.5 -0.8 N.D. -1.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.2 -1.4 N.D. +0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 -1.0 N.D. -0.5 N.D.
phage Wcs_1 -0.8 +0.3 N.D. -0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -1.7 N.D. +0.5 N.D.
nophagecontrol Only library +0.2 -0.4 N.D. -0.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 -1.1 N.D. +0.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.3 N.D. -1.1 N.D.
stress Gentamicin 0.008 mg/ml +0.2 -1.6 N.D. +0.4 N.D.
carbon source L-Isoleucine (C) -0.0 -1.3 N.D. +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -1.0 N.D. +0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.7 N.D. +0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +0.5 N.D. -0.6 N.D.
seeds Growth on radish seeds for 72 hours -0.2 +0.9 N.D. -0.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.5 N.D. -0.7 N.D.
phage P. simiae OR Antrim MOI 0.1 -0.2 +1.1 N.D. -0.5 N.D.
phage P. simiae OR Antrim MOI 0.1 -0.3 +0.9 N.D. -0.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.3 +0.7 N.D. -0.5 N.D.
phage P. simiae OR Antrim MOI 10 -0.1 +1.1 N.D. -0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.4 -0.0 N.D. +1.2 N.D.
carbon source L-Valine (C) -0.7 +0.7 N.D. +0.9 N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB +0.3 +0.9 N.D. -0.2 N.D.
stress Doxycycline hyclate 0.001 mg/ml -0.2 +1.0 N.D. +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.7 +0.6 N.D. +0.8 N.D.
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