Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_18635 and PS417_18640 are separated by 39 nucleotidesPS417_18640 and PS417_18645 are separated by 15 nucleotidesPS417_18645 and PS417_18650 are separated by 182 nucleotidesPS417_18650 and PS417_18655 are separated by 78 nucleotides PS417_18635: PS417_18635 - 4-hydroxybenzoyl-CoA thioesterase, at 4,051,169 to 4,051,603 _18635 PS417_18640: PS417_18640 - hydrolase, at 4,051,643 to 4,052,476 _18640 PS417_18645: PS417_18645 - TetR family transcriptional regulator, at 4,052,492 to 4,053,031 _18645 PS417_18650: PS417_18650 - tRNA-Glu, at 4,053,214 to 4,053,289 _18650 PS417_18655: PS417_18655 - tRNA-Ala, at 4,053,368 to 4,053,443 _18655
Group Condition PS417_18635 PS417_18640 PS417_18645 PS417_18650 PS417_18655
rhizosphere rhizosphere sample 1; outgrowth in LB -1.0 -0.9 N.D. N.D. N.D.
liquid stress Scopoletin 3 mM; liquid stress -2.0 +0.1 N.D. N.D. N.D.
soil soil sample 7; outgrowth in LB -1.5 -0.1 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.6 -0.9 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.2 -0.3 N.D. N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.1 -0.4 N.D. N.D. N.D.
stress Cisplatin 0.01 mg/ml -1.3 -0.1 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.9 -0.5 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 -0.1 N.D. N.D. N.D.
carbon source L-Valine (C) -1.2 -0.0 N.D. N.D. N.D.
rhizosphere rhizosphere sample 2; outgrowth in LB -1.5 +0.4 N.D. N.D. N.D.
phage JP1 MOI 10 +0.3 -1.1 N.D. N.D. N.D.
phage Wcs_1 -1.1 +0.3 N.D. N.D. N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.0 +0.3 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.4 -1.0 N.D. N.D. N.D.
agar plate interaction control Taped volatile agar plate with no fungus -1.0 +0.6 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 -1.0 N.D. N.D. N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 +0.6 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 -0.6 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 +0.9 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.4 -0.1 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.0 +0.3 N.D. N.D. N.D.
phage P. simiae Grant Run MOI 0.1 +0.9 +0.4 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.9 +2.3 N.D. N.D. N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x +1.2 +0.3 N.D. N.D. N.D.
carbon source 2'-Deoxyinosine 5 mM (C) +1.5 +0.1 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 +1.1 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +1.8 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.7 +0.2 N.D. N.D. N.D.
nophagecontrol Only library +1.8 +0.6 N.D. N.D. N.D.
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